Medical Microbiology and Immunology

, Volume 203, Issue 5, pp 333–340 | Cite as

Molecular typing of clinical Cryptococcus neoformans isolates collected in Germany from 2004 to 2010

  • Andrea Sanchini
  • Ilka McCormick Smith
  • Ludwig Sedlacek
  • Roman Schwarz
  • Kathrin Tintelnot
  • Volker Rickerts
Original Investigation

Abstract

Cryptococcosis is a fungal infection mostly caused by Cryptococcus neoformans. We identified agents of cryptococcosis diagnosed in Germany from 2004 to 2010. We used multi-locus sequence typing (MLST) to understand the molecular epidemiology of cryptococcosis. Sero- and mating types of individual patient isolates were determined by PCR. MLST was performed using the seven-locus scheme. Allele and nucleotide diversity was calculated for each locus of C. neoformans var. grubii and C. neoformans var. neoformans. Phylogenetic relations were assessed by dendrograms. Clinical data were compared between infections caused by the two variants. We studied 101 isolates. Eight were identified as hybrids (8 %). All non-hybrids were of the α mating type. Among 78 C. neoformans var. grubii (77 %), 16 sequence types (STs) were identified including three novel STs. They clustered in four groups, previously isolated in Asia, Europe or worldwide. Among 15 C. neoformans var. neoformans (15 %), 10 STs were identified, without clustering. These isolates showed higher allele, and nucleotide diversity compared with C. neoformans var. grubii. C. neoformans var. neoformans was more likely to cause soft-tissue infections (3/9, 33 vs. 1/63, 2 %, p = 0.005) and to affect non-AIDS patients (7/14, 50 vs. 15/76, 20 %, p = 0.036). C. neoformans var. grubii is the predominant agent of cryptococcosis in Germany. MLST suggests that a part of these cases are acquired abroad by immigrants or tourists. C. neoformans var. neoformans isolates represent a greater genetic diversity and are associated with more variable clinical presentations.

Keywords

Cryptococcus neoformans var. grubii Cryptococcus neoformans var. neoformanMLST Cryptococcosis Molecular epidemiology AD hybrids 

Supplementary material

430_2014_341_MOESM1_ESM.docx (98 kb)
Supplementary material 1 (DOCX 98 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Andrea Sanchini
    • 1
    • 2
  • Ilka McCormick Smith
    • 1
  • Ludwig Sedlacek
    • 3
  • Roman Schwarz
    • 4
  • Kathrin Tintelnot
    • 1
  • Volker Rickerts
    • 1
  1. 1.Robert Koch-InstitutBerlinGermany
  2. 2.European Public Health Microbiology (EUPHEM) Training ProgrammeEuropean Centre for Disease Prevention and Control (ECDC)StockholmSweden
  3. 3.Institute for Medical Microbiology and Hospital EpidemiologyHannover Medical SchoolHannoverGermany
  4. 4.MVZ Labor Dr. Stein+KollegenMönchengladbachGermany

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