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Medical Microbiology and Immunology

, Volume 203, Issue 1, pp 25–33 | Cite as

Anti-biofilm activity of low-molecular weight chitosan hydrogel against Candida species

  • A. Silva-DiasEmail author
  • A. Palmeira-de-Oliveira
  • I. M. Miranda
  • J. Branco
  • L. Cobrado
  • M. Monteiro-Soares
  • J. A. Queiroz
  • C. Pina-Vaz
  • A. G. Rodrigues
Original Investigation

Abstract

Candida invasive infections have increased in frequency during the last decades. Such infections are often associated to medical indwelling devices like central venous catheter. The recurrent nature and difficulties in the treatment of these infections are often related to biofilm formation. The objective of this study was to investigate the anti-biofilm activity of low-molecular weight chitosan hydrogel (LMWCH), a natural biopolymer obtained from the N-deacylation of crustacean chitin, upon clinical relevant Candida species. The in vitro ability of LMWCH to impair biofilm formation and to disorganize a preformed biofilm was tested in polystyrene microplates and quantified by the semi quantitative XTT assay and by the crystal violet assay. LMWCH in vivo efficacy as a coating for medical indwelling devices was evaluated for the first time for Candida parapsilosis, using a mouse subcutaneous foreign body model using polyurethane catheter segments. Scanning electron microscopy was used to access biofilm architecture after LMWCH treatment. We found that LMWCH efficiently impaired biofilm formation of all Candida species, also promoting biofilm disaggregation. Most importantly, LMWCH was able to significantly inhibit biofilm formation by C. parapsilosis in an in vivo catheter mouse model. SEM images showed biofilm collapsed cells compatible with membrane damage, suggesting that this could be one of the possible mechanisms underlying biofilm impairment. LMWCH revealed to be a promising compound for treatment of candidiasis or its prevention through medical device coating.

Keywords

Biofilm Candidiasis Catheter-related bloodstream infections Central venous catheter 

Notes

Acknowledgments

We are grateful to Dr. Daniela Silva from Materials Center of the University of Porto (CEMUP), for the helpful assistance with the scanning electron microscopy. We are also grateful to “Fundação para a Ciência e Tecnologia (FCT)” for financial support. This work was supported by the project: “QUITORAL: Desenvolvimento de novas formulações de quitosanos com aplicação em medicina oral”—QREN—ADI 3474, financed by “European Regional Development Fund (ERDF)”. This work was also supported by “Fundação para a Ciência e Tecnologia (FCT)”, by Ph.D. Grant [SFRH/BD/44896/2008 to ASD]. IMM is supported by FCT Ciência 2008 and cofinanced by the European Social Fund.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

Animal studies were approved by Direcção-Geral de Alimentação e Veterinária (DGAV), the Portuguese National Authority for Animal Health.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • A. Silva-Dias
    • 1
    • 2
    Email author
  • A. Palmeira-de-Oliveira
    • 3
  • I. M. Miranda
    • 1
    • 2
  • J. Branco
    • 1
  • L. Cobrado
    • 1
    • 2
    • 4
  • M. Monteiro-Soares
    • 5
  • J. A. Queiroz
    • 3
  • C. Pina-Vaz
    • 1
    • 2
    • 6
  • A. G. Rodrigues
    • 1
    • 2
    • 4
  1. 1.Department of Microbiology, Faculty of MedicineUniversity of PortoPortoPortugal
  2. 2.Cardiovascular Research and Development Unit, Faculty of MedicineUniversity of PortoPortoPortugal
  3. 3.Faculty of Health Sciences, CICS-UBI, Health Sciences Research CenterUniversity of Beira InteriorCovilhãPortugal
  4. 4.Burn Unit and Department of Plastic and Reconstructive SurgeryHospital S. JoãoPortoPortugal
  5. 5.CIDES, Department of Information and Decision Sciences in Health, Faculty of MedicineUniversity of PortoPortoPortugal
  6. 6.Department of MicrobiologyHospital S. JoãoPortoPortugal

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