Infection of human brain vascular pericytes (HBVPs) by Bartonella henselae
Angiogenesis is an important physiological and pathological process. Bartonella is the only genus of bacteria known to induce pathological angiogenesis in the mammalian host. Bartonella-induced angiogenesis leads to the formation of vascular tumors including verruga peruana and bacillary angiomatosis. The mechanism of Bartonella-induced angiogenesis is not completely understood. Pericytes, along with endothelial cells, play an important role in physiological angiogenesis, and their role in tumor angiogenesis has been extensively studied. Abnormal signaling between endothelial cells and pericytes contributes to tumor angiogenesis and metastasis; however, the role of pericytes in Bartonella-induced angiogenesis is not known. In this study, after infecting human brain vascular pericytes (HBVPs) with Bartonella henselae, we found that these bacteria were able to invade HBVPs and that bacterial infection resulted in decreased pericyte proliferation and increased pericyte production of vascular endothelial growth factor (VEGF) when compared to the uninfected control cells. In the context of pathological angiogenesis, reduced pericyte coverage, accompanied by increased VEGF production, may promote endothelial cell proliferation and the formation of new vessels.
KeywordsBlood vessels Bacteria Cytokines Vascular endothelial growth factor Pericyte coverage
We would like to thank Dr. Maria Correa and Ms. Elizabeth Pultorak for assistance with the statistical analysis, and Dr. Shila Nordone and Ms. Barbara Hegarty for critically reading the manuscript. This study was funded by American Kennel Club-Canine Health Foundation AKORN grant 01531A. We also thank Bayer Animal Health Foundation for providing stipend support for Dr. Mrudula Varanat who was a graduate student in Intracellular Pathogens Laboratory, North Carolina State University at the time this research was performed.
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