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Medical Microbiology and Immunology

, Volume 201, Issue 1, pp 25–35 | Cite as

The protective Th1 response in mice is induced in the T-cell zone only three weeks after infection with Leishmania major and not during early T-cell activation

  • Julia Barthelmann
  • Julia Nietsch
  • Maike Blessenohl
  • Tamas Laskay
  • Ger van Zandbergen
  • Jürgen Westermann
  • Kathrin KaliesEmail author
Original Investigation

Abstract

The protozoan parasite Leishmania spp. causes clinical pictures ranging in severity from spontaneously healing skin ulcers to systemic disease. The immune response associated with healing involves the differentiation of IFNγ-producing Th1 cells, whereas the non-healing phenotype is associated with IL4-producing Th2 cells. The widespread assumption has been that the T-cell differentiation that leads to a healing or non-healing phenotype is established at the time of T-cell activation early after infection. By selectively analyzing the expression of cytokine genes in the T-cell zones of lymph nodes of resistant (Th1) C57BL/6 mice and susceptible (Th2) BALB/c mice during an infection with Leishmania major in vivo, we show that the early T-cell response does not differ between C57BL/6 mice and BALB/c mice. Instead, Th1/Th2 polarization appears suddenly 3 weeks after infection. At the same time point, the number of parasites increases in lymph nodes of both mouse strains, but about 100-fold more in susceptible BALB/c mice. We conclude that the protective Th1 response in C57BL/6 mice is facilitated by the capacity of their innate effector cells to keep parasite numbers at low levels.

Keywords

Leishmania major infection Th1/Th2 cell response In vivo laser microdissection 

Notes

Acknowledgments

We thank L. Gutjahr, P. Lau, M.-L. Leppin, K. von Lingelsheim, and S. Möller for technical assistance. Supported by the Deutsche Forschungsgemeinschaft (SFB 654, C4) and the Excellence Cluster “Inflammation at Interfaces” (DFG EXC 306/1) (to JW).

Supplementary material

430_2011_201_MOESM1_ESM.doc (76 kb)
Supplementary material 1 (DOC 75 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Julia Barthelmann
    • 1
  • Julia Nietsch
    • 1
  • Maike Blessenohl
    • 1
  • Tamas Laskay
    • 2
  • Ger van Zandbergen
    • 2
    • 3
  • Jürgen Westermann
    • 1
  • Kathrin Kalies
    • 1
    Email author
  1. 1.Center for Structural and Cell Biology in Medicine, Institute of AnatomyUniversity of LübeckLübeckGermany
  2. 2.Institute for Medical Microbiology and HygieneUniversity of LübeckLübeckGermany
  3. 3.Department of Medical Microbiology and HygieneUniversity of UlmUlmGermany

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