Medical Microbiology and Immunology

, Volume 199, Issue 1, pp 11–25 | Cite as

Cellular immune response to Mycobacterium tuberculosis-specific antigen culture filtrate protein-10 in south India

  • Madhan Kumar
  • Jagadish C. Sundaramurthi
  • Narinder K. Mehra
  • Gurvinder Kaur
  • Alamelu Raja
Original Investigation

Abstract

The Mycobacterium tuberculosis (M. tuberculosis)-specific culture filtrate protein-10 (CFP-10) is highly recognized by M. tuberculosis infected subjects. In the present study, the proliferative response and IFN-γ secretion was found for C-terminal peptides of the protein (Cfp651–70, Cfp761–80, Cfp871–90, and Cfp981–100). The alleles HLA DRB1 *04 and HLA DRB1 *10 recognized the C-terminal peptides Cfp7, Cfp8, and Cfp9 in HHC. Cfp6 was predominantly recognized by the alleles HLA DRB1 *03 and HLA DRB1 *15 by PTB. The minimal nonameric epitopes from the C-terminal region were CFP-1056–64 and CFP-1076–84. These two peptides deserve attention for inclusion in a vaccine against tuberculosis in this region.

Keywords

CFP-10 HLA analysis Epitope mapping Overlapping peptides Proliferation Interferon gamma In silico CFP-10 IL-4 ELISA Tuberculosis 

Notes

Acknowledgments

Mr. Madhan Kumar is a recipient of Senior Research Fellowship from Council of Scientific and Industrial Research (CSIR), New Delhi, India. Overlapping peptides of CFP-10 were a kind gift from Dr. Thomas B Nutman, NIAID/NIH, USA (under ICER/TRC program). CFP-10 whole protein was a gift from Dr. Pawan Sharma, ICGEB (International Centre for Genetic Engineering and Biotechnology), New Delhi. We would like to thank Mr. Anbalagan and Mr. Murugesh for their help in acquiring samples in flow cytometry. We are grateful to patients, healthy household contacts, and treated patients who participated in this study and gave blood. The help rendered by the RNTCP staff Mrs. Kasthuri and Mrs. Thilagavathi in recruiting patients is kindly acknowledged. This project was not a funded project.

Conflict of interest statement

None.

Supplementary material

430_2009_129_MOESM1_ESM.doc (170 kb)
Supplementary material 1 (DOC 170 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Madhan Kumar
    • 1
  • Jagadish C. Sundaramurthi
    • 2
  • Narinder K. Mehra
    • 3
  • Gurvinder Kaur
    • 3
  • Alamelu Raja
    • 1
  1. 1.Department of ImmunologyTuberculosis Research Centre (ICMR)Chetput, ChennaiIndia
  2. 2.Biomedical Informatics CentreTuberculosis Research Centre (ICMR)Chetput, ChennaiIndia
  3. 3.Department of Transplant Immunology and ImmunogeneticsAll India Institute of Medical SciencesNew DelhiIndia

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