Comparison of performance in two diagnostic methods for tuberculosis infection

  • Kazue Higuchi
  • Yoshiko Kawabe
  • Satoshi Mitarai
  • Takashi Yoshiyama
  • Nobuyuki Harada
  • Toru Mori
Original Investigation


Performance of two diagnoses, T-SPOT®.TB (T-SPOT) and QuantiFERON-TB® Gold (QFT-G), was compared in Japanese subjects. Forty-seven confirmed tuberculosis patients and eighty-four healthy subjects were recruited. All samples were assessed for both T-SPOT and QFT-G, and the sensitivities and the specificities were compared between two methods. The sensitivity was 100% for T-SPOT, and 87.2% for QFT-G. The specificity was 83.3 and 98.8%, respectively. The overall agreement of two tests was substantially high (Kappa coefficient = 0.671). The sensitivity of T-SPOT appeared to be higher than that of QFT-G, whereas the specificity of T-SPOT was significantly lower than that of QFT-G. The difference in the performance between T-SPOT and QFT-G and biological relevance of each system in diagnosing M. tuberculosis infection should be further explored.


Interferon-gamma Tuberculosis Diagnosis ESAT-6 CFP-10 


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Kazue Higuchi
    • 1
  • Yoshiko Kawabe
    • 2
  • Satoshi Mitarai
    • 1
  • Takashi Yoshiyama
    • 3
  • Nobuyuki Harada
    • 1
  • Toru Mori
    • 1
    • 4
  1. 1.Immunology DivisionThe Research Institute of Tuberculosis, Japan Anti-Tuberculosis AssociationKiyoseJapan
  2. 2.National Hospital Organization Tokyo HospitalKiyoseJapan
  3. 3.Fukujuji HospitalKiyoseJapan
  4. 4.Leprosy Research CenterNational Institute of Infectious DiseasesHigashimurayamaJapan

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