Medical Microbiology and Immunology

, Volume 198, Issue 1, pp 5–11 | Cite as

Evaluation of a recombinant BCG expressing antigen Ag85B and PPE protein Rv3425 from DNA segment RD11 of Mycobacterium tuberculosis in C57BL/6 mice

  • Jiu ling Wang
  • Ya qing Qie
  • Bing dong Zhu
  • Hong mei Zhang
  • Ying Xu
  • Qing zhong Wang
  • Jia zhen Chen
  • Wei Liu
  • Hong hai WangEmail author
Original Investigation


Antigen 85B (Ag85B) is an important immunodominant antigen of Mycobacterium tuberculosis, and is a very promising vaccine candidate molecule. Rv3425 is a member of the subgroup 3 of the PPE family, which does not exist in all BCG strains. In this study we constructed a new rBCG which included this united gene (Ag85B-Rv3425). The level of antigen-stimulated T cells expressing IFN-γ was significantly higher in the C57BL/6 mice vaccinated with rBCG::Ag85B-Rv3425 than with BCG. In addition, the sera from mice immunized with rBCG::Ag85B-Rv3425 revealed an increase in the specific immunoglobulin G titers than that from mice immunized with BCG. Antigen specific IgG subclass analysis showed that rBCG::Ag85B-Rv3425 tended to facilitate IgG2a production, suggesting enhancement of predominant Th1 response which in turn may facilitate increased production of protective IFN-γ. These results suggested that this rBCG::Ag85B-Rv3425 could be a strong vaccine candidate for further study.


Mycobacterium tuberculosis Vaccine Ag85B Rv3425 



This work was supported by the National High Technology Research and Development Program of China (863 Program) (2006AA02Z445 and 2006AA02Z420).


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jiu ling Wang
    • 1
  • Ya qing Qie
    • 1
  • Bing dong Zhu
    • 2
  • Hong mei Zhang
    • 3
  • Ying Xu
    • 1
  • Qing zhong Wang
    • 1
  • Jia zhen Chen
    • 1
  • Wei Liu
    • 1
  • Hong hai Wang
    • 1
    • 4
    Email author
  1. 1.State Key Laboratory of Genetic Engineering, Institute of GeneticsFudan UniversityShanghaiPeople’s Republic of China
  2. 2.Lanzhou Center for Tuberculosis ResearchLanzhou UniversityLanzhouPeople’s Republic of China
  3. 3.Henan Institute of Medical SciencesHenanPeople’s Republic of China
  4. 4.Institute of Genetics, School of Life ScienceFudan UniversityShanghaiPeople’s Republic of China

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