Medical Microbiology and Immunology

, Volume 193, Issue 4, pp 181–187

Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus

  • Andreas Lucht
  • Roland Grunow
  • Christian Otterbein
  • Peggy Möller
  • Heinz Feldmann
  • Stephan Becker
Original Investigation

Abstract

Ebola virus (EBOV) causes severe outbreaks of Ebola hemorrhagic fever in endemic regions of Africa and is considered to be of impact for other parts of the world as an imported viral disease. To develop a new diagnostic test, monoclonal antibodies to EBOV were produced from mice immunized with inactivated EBOV species Zaire. Antibodies directed against the viral glycoprotein GP were characterized by ELISA, Western blot and immunofluorescence analyses. An antigen capture ELISA was established, which is specific for EBOV-Zaire and shows a sensitivity of approximately 103 plaque-forming units/ml. Since the ELISA is able to detect even SDS-inactivated EBOV in spiked human sera, it could complement the existing diagnostic tools in the field and in routine laboratories where high containment facilities are not available.

Keywords

Ebola virus Ebola hemorrhagic fever Monoclonal antibodies Antigen capture ELISA 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Andreas Lucht
    • 1
  • Roland Grunow
    • 1
  • Christian Otterbein
    • 1
    • 3
  • Peggy Möller
    • 2
  • Heinz Feldmann
    • 2
    • 4
  • Stephan Becker
    • 2
  1. 1.Bundeswehr Institute of MicrobiologyMunichGermany
  2. 2.Institute of VirologyPhilipps-University MarburgMarburgGermany
  3. 3.Authority of the district of RosenheimPublic Veterinary OfficeBad AiblingGermany
  4. 4.Special Pathogens Program, National Microbiology Laboratory, Health CanadaCanadian Science Centre for Human and Animal HealthWinnipegCanada

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