Anatomy and Embryology

, Volume 196, Issue 6, pp 457–463 | Cite as

Expression of lymphocyte antigenic determinants in developing gut-associated lymphoid tissue of the sea bass Dicentrarchus labrax (L.)

  • Simona Picchietti
  • Francesca Renata Terribili
  • Lucia Mastrolia
  • Giuseppe Scapigliati
  • L. Abelli
ORIGINAL ARTICLE

Abstract

 The monoclonal antibodies DLT15 and DLIg3, which recognize antigenic determinants expressed by T cells and Ig-bearing cells, respectively, allowed the development of gut-associated lymphoid tissue of the teleost fish Dicentrarchus labrax (L.) to be studied. DLT15- immunoreactive cells were first detected in the epithelium of the stomach and intestine at day 30 post-hatching of fish maintained at 16° C. At that age, positive cells were found only in the thymus. Between day 44 and day 81 post-hatching, DLT15-immunoreactive cells became numerous, both in and under the gut epithelium. A gradient in the number of lymphocytes was present, concentrating them towards the anus. Until day 81 post-hatching, DLIg3-immunoreactive cells were not found in the gut, although they were present in the kidney, spleen and thymus earlier. Infrequent Ig-bearing cells were found in the gut mucosa of 1-year-old sea bass. This study showed that the gut-associated lymphoid tissue developed earlier than other lymphoid compartments. It also provided evidence of the predominance of T cells in the gut immune system of the sea bass.

Key words T cell B cell Monoclonal antibodies Gut Fish 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Simona Picchietti
    • 1
  • Francesca Renata Terribili
    • 1
  • Lucia Mastrolia
    • 1
  • Giuseppe Scapigliati
    • 1
  • L. Abelli
    • 1
  1. 1.Dipartimento di Scienze Ambientali, Università della Tuscia, Via S. Camillo de Lellis, blocco D, I-01100 Viterbo, Italy Fax: +761-357179; e-mail: labelli@unitus.itIT

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