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Hippocampal LTP modulation and glutamatergic receptors following vestibular loss

  • B. Truchet
  • A. Benoit
  • F. Chaillan
  • P. F. Smith
  • B. Philoxene
  • M. Guillamin
  • B. Poucet
  • A. Coquerel
  • Stephane BesnardEmail author
Original Article
  • 67 Downloads

Abstract

Vestibular dysfunction strongly impairs hippocampus-dependent spatial memory performance and place cell function. However, the hippocampal encoding of vestibular information at the synaptic level, remains sparsely explored and controversial. We investigated changes in in vivo long-term potentiation (LTP) and NMDA glutamate receptor (NMDAr) density and distribution after bilateral vestibular lesions (BVL) in adult rats. At day 30 (D30) post-BVL, the LTP of the population spike recorded in the dentate gyrus (DG) was higher in BVL rats, for the entire 3 h of LTP recording, while no difference was observed in the fEPSP slope. However, there was an increase in EPSP–spike (E–S) potentiation in lesioned rats. NMDArs were upregulated at D7 and D30 predominantly within the DG and CA1. At D30, we observed a higher NMDAr density in the left hippocampus. NMDArs were overexpressed on both neurons and non-neuronal cells, suggesting a decrease of the entorhinal glutamatergic inputs to the hippocampus following BVL. The EPSP–spike (E–S) potentiation increase was consistent with the dorsal hippocampus NMDAr upregulation. Such an increase could reflect a non-specific enhancement of synaptic efficacy, leading to a disruption of memory encoding, and therefore might underlie the memory deficits previously reported in rats and humans following vestibular loss.

Keywords

NMDA receptors Dentate gyrus E–S potentiation In vivo RRID: AB_670215 AB_1841228 

Notes

Acknowledgements

The authors would like to thank Mrs Guillemette Gaucquelin-Koch, the Centre National de la Recherche Spatiale, the People Programme of the European Union’s Seventh Framework Programme FP7/2007-2013/through REA (Grant number: 318980) and the Normandy Region for their financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures were conducted in accordance with the European Communities Council Directive 86/6609/EEC, as well as French legislation. The protocol was approved by the regional ethical committee (Comité d’Ethique Normandie en Matière d’Expérimentation Animale, CENOXEMA, number assigned 0412-01).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • B. Truchet
    • 1
  • A. Benoit
    • 2
  • F. Chaillan
    • 1
  • P. F. Smith
    • 3
  • B. Philoxene
    • 2
  • M. Guillamin
    • 4
  • B. Poucet
    • 1
  • A. Coquerel
    • 5
  • Stephane Besnard
    • 2
    Email author
  1. 1.Aix Marseille Univ, CNRS, LNC UMR 7291, FR 3C FR 3512MarseilleFrance
  2. 2.Université de Normandie, INSERM U 1075 COMETECaenFrance
  3. 3.Department of Pharmacology and Toxicology, School of Biomedical SciencesUniversity of OtagoDunedinNew Zealand
  4. 4.Université de Normandie, SF 4206 ICORE, UMR BioTICLA 1199CaenFrance
  5. 5.Département de PharmacologieCHU de CaenCaenFrance

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