The caudo-ventral pallium is a novel pallial domain expressing Gdf10 and generating Ebf3-positive neurons of the medial amygdala
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In rodents, the medial nucleus of the amygdala receives direct inputs from the accessory olfactory bulbs and is mainly implicated in pheromone-mediated reproductive and defensive behaviors. The principal neurons of the medial amygdala are GABAergic neurons generated principally in the caudo-ventral medial ganglionic eminence and preoptic area. Beside GABAergic neurons, the medial amygdala also contains glutamatergic Otp-expressing neurons cells generated in the lateral hypothalamic neuroepithelium and a non-well characterized Pax6-positive population. In the present work, we describe a novel glutamatergic Ebf3-expressing neuronal subpopulation distributed within the periphery of the postero-ventral medial amygdala. These neurons are generated in a pallial domain characterized by high expression of Gdf10. This territory is topologically the most caudal tier of the ventral pallium and accordingly, we named it Caudo-Ventral Pallium (CVP). In the absence of Pax6, the CVP is disrupted and Ebf3-expressing neurons fail to be generated. Overall, this work proposes a novel model of the neuronal composition of the medial amygdala and unravels for the first time a new novel pallial subpopulation originating from the CVP and expressing the transcription factor Ebf3.
KeywordsTelencephalon Ventral pallium Medial amygdala Gdf10 Ebf3 Pax6 Caudo-ventral pallium
Basolateral complex of the amygdala
Basomedial nucleus of the amygdala
Cranial bone progenitors
Caudal ganglionic eminence
In situ hybridization
Lateral ganglionic eminence.
Lateral thalamic eminence.
Posterodorsal medial Amygdala
Posteroventral medial amygdala
Medial ganglionic eminence
Medial thalamic eminence
Preoptic amygdala stream
Paraventricular hypothalamic domain
Superficial layer of the medial nucleus
Ventropallial amygdalopiriform area
Ventrolateral caudal pallium
Zona limitants intrathalamica
We thank Dr. F. Vaccarino and L. Tomasini for Otp antibody, and Dr. A. Mansouri for the Pax6KO colony. Special thanks to Dr. M. Bertacchi for sharing the Pax6KO colony.
This work was supported by Grants of the French National Research Agency (Agence Nationale de la Recherche; ANR) [ANR-13-BSV4-0011] and by the French Government through the ‘Investments for the Future’ LABEX SIGNALIFE [ANR-11-LABX-0028-01] to M.S., by the Spanish Government (BFU2007-60263 and BFU2010-17305) to A.F, and by the Medical Research Council (MR/K013750/1) to T.T. N.R.-R. is funded by a postdoctoral fellowship from the Ville de Nice, France (“Aide Individuelle aux Jeunes Chercheurs 2016”).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. The care and handling of mice prior or during the experimental procedures followed European Union rules (2010/63/UE) and were approved by the Animal Care and Use Committees of Spain and France.
Informed consent was obtained from all individual participants included in the study.
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