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Brain Structure and Function

, Volume 223, Issue 5, pp 2259–2268 | Cite as

Extrasynaptic homomeric glycine receptors in neurons of the rat trigeminal mesencephalic nucleus

  • Jin Young Bae
  • Jae Sik Lee
  • Sang Jin Ko
  • Yi Sul Cho
  • Jong-Cheol Rah
  • Hee Jung Cho
  • Mae Ja Park
  • Yong Chul BaeEmail author
Original Article

Abstract

The neurons in the trigeminal mesencephalic nucleus (Vmes) innervate jaw-closing muscle spindles and periodontal ligaments, and play a crucial role in the regulation of jaw movements. Recently, it was shown that many boutons that form synapses on them are immunopositive for glycine (Gly+), suggesting that these neurons receive glycinergic input. Information about the glycine receptors that mediate this input is needed to help understand the role of glycine in controlling Vmes neuron excitability. For this, we investigated the expression of glycine receptor subunit alpha 3 (GlyRα3) and gephyrin in neurons in Vmes and the trigeminal motor nucleus (Vmo), and the Gly+ boutons that contact them by light- and electron-microscopic immunocytochemistry and quantitative ultrastructural analysis. The somata of the Vmes neurons were immunostained for GlyRα3, but not gephyrin, indicating expression of homomeric GlyR. The immunostaining for GlyRα3 was localized away from the synapses in the Vmes neuron somata, in contrast to the Vmo neurons, where the staining for GlyRα3 and gephyrin were localized at the subsynaptic zones in somata and dendrites. Additionally, the ultrastructural determinants of synaptic strength, bouton volume, mitochondrial volume, and active zone area, were significantly smaller in Gly+ boutons on the Vmes neurons than in those on the Vmo neurons. These findings support the notion that the Vmes neurons receive glycinergic input via putative extrasynaptic homomeric glycine receptors, likely mediating a slow, tonic modulation of the Vmes neuron excitability.

Keywords

Mesencephalic trigeminal nucleus Glycine receptor Homomeric Jaw movement Ultrastructure Immunohistochemistry 

Notes

Acknowledgements

The authors sincerely thank Dr. Juli Valtschanoff for helpful discussion and careful reading of the manuscript.

Funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2017R1A5A2015391, NRF-2017R1A2B2003561).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Anatomy and Neurobiology, School of Medicine and DentistryKyungpook National UniversityDaeguSouth Korea
  2. 2.Korea Brian Research InstituteDaeguSouth Korea

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