Brain Structure and Function

, Volume 221, Issue 7, pp 3445–3473

Distribution of oxytocin and co-localization with arginine vasopressin in the brain of mice

  • Marcos Otero-García
  • Carmen Agustín-Pavón
  • Enrique Lanuza
  • Fernando Martínez-García
Original Article

DOI: 10.1007/s00429-015-1111-y

Cite this article as:
Otero-García, M., Agustín-Pavón, C., Lanuza, E. et al. Brain Struct Funct (2016) 221: 3445. doi:10.1007/s00429-015-1111-y

Abstract

Oxytocin (OT) and vasopressin (AVP) play a major role in social behaviours. Mice have become the species of choice for neurobiology of social behaviour due to identification of mouse pheromones and the advantage of genetically modified mice. However, neuroanatomical data on nonapeptidergic systems in mice are fragmentary, especially concerning the central distribution of OT. Therefore, we analyse the immunoreactivity for OT and its neurophysin in the brain of male and female mice (strain CD1). Further, we combine immunofluorescent detection of OT and AVP to locate cells co-expressing both peptides and their putative axonal processes. The results indicate that OT is present in cells of the neurosecretory paraventricular (Pa) and supraoptic hypothalamic nuclei (SON). From the anterior SON, OTergic cells extend into the medial amygdala, where a sparse cell population occupies its ventral anterior and posterior divisions. Co-expression of OT and AVP in these nuclei is rare. Moreover, a remarkable OTergic cell group is found near the ventral bed nucleus of the stria terminalis (BST), distributed between the anterodorsal preoptic nucleus and the nucleus of anterior commissure (ADP/AC). This cell group, the rostral edge of the Pa and the periventricular hypothalamus display frequent OT + AVP double labelling, with a general dominance of OT over AVP immunoreactivity. Fibres with similar immunoreactivity profile innervate the accumbens shell and core, central amygdala and portions of the intervening BST. These data, together with data in the literature on rats, suggest that the projections of ADP/AC nonapeptidergic cells onto these brain centres could promote pup-motivated behaviours and inhibit pup avoidance during motherhood.

Keywords

Amygdala Bed nucleus of the stria terminalis Accumbens Maternal behaviour Sociosexual brain Neurosecretory cells 

Abbreviations

10N

Dorsal motor nucleus of the vagus

12N

Hypoglossal nucleus

3V

Third ventricle

4n

Trochlear nerve or its root

4V

4th ventricle

A1

A1 noradrenaline cells

AC

Anterior commissural nucleus

AC

Anterior commissural nucleus

aca

Anterior commissure, anterior part

Acb

Accumbens nucleus

AcbC

Accumbens nucleus, core

AcbSh

Accumbens nucleus, shell

ACo

Anterior cortical amygdaloid nucleus

acp

Anterior commissure, posterior part

AD

Anterodorsal thalamic nucleus

ADP

Anterodorsal preoptic nucleus

AHA

Anterior hypothalamic area, anterior part

AHP

Anterior hypothalamic area, posterior part

AIP

Agranular insular cortex, posterior part

AM

Anteromedial thalamic nucleus

Amb

Ambiguus nucleus

AN

Accessory nuclei

AP

Area postrema

APir

Amygdalopiriform transition area

Aq

Aqueduct (Sylvius)

Arc

Arcuate hypothalamic nucleus

AStr

Amygdalostriatal transition area

AV

Anteroventral thalamic nucleus

AVP

Arginine vasopressin

AVPe

Anteroventral periventricular nucleus

AVP-ir

Arginine vasopressin immunoreactive

AVV

Anteroventral thalamic nucleus, ventral part

BAC

Bed nucleus of the anterior commissure

BAOT

Bed nucleus of the accessory olfactory

Bar

Barrington’s nucleus

BLA

Basolateral amygdaloid nucleus, anterior

BLV

Basolateral amygdaloid nucleus, ventral

BMA

Basomedial amygdaloid nucleus, anterior

BST

Bed nucleus of the stria terminalis

BSTA

Bed nucleus of the stria terminalis, anterior part

BSTIA

Bed nucleus of the stria terminalis, intraamygdaloid division

BSTLD

Bed nucleus of the stria terminalis, lateral division, dorsal part

BSTLP

Bed nucleus of the stria terminalis, lateral division, posterior part

BSTLV

Bed nucleus of the stria terminalis, lateral division, ventral part

BSTMA

Bed nucleus of the stria terminalis, medial division, anterior part

BSTMP

Bed nucleus of the stria terminalis, medial division, posterior part

BSTMPI

Bed nucleus of the stria terminalis, medial division, posterointermediate part

BSTMPL

Bed nucleus of the stria terminalis, medial division, posterolateral part

BSTMPM

Bed nucleus of the stria terminalis, medial division, posteromedial part

BSTMV

Bed nucleus of the stria terminalis, medial division, ventral part

BSTS

Bed nucleus of the stria terminalis, supracapsular part

cc

Corpus callosum

CC

Central canal

Ce

Central amygdaloid nucleus

CeC

Central amygdaloid nucleus, capsular part

CeL

Central amygdaloid nucleus, lateral division

CeM

Central amygdaloid nucleus, medial division

CeMAD

Central amygdaloid nucleus, medial division, anterodorsal part

CeMAV

Central amygdaloid nucleus, medial division, anteroventral part

CGPn

Central grey of the pons

Cl

Claustrum

CL

Centrolateral thalamic nucleus

CM

Central medial thalamic nucleus

cp

Cerebral peduncle, basal part

CPu

Caudate putamen (striatum)

cst

Commissural stria terminalis

Cu

Cuneate nucleus

CxA

Cortex–amygdala transition zone

D3V

Dorsal third ventricle

DEn

Dorsal endopiriform nucleus

DG

Dentate gyrus

DLPAG

Dorsolateral periaqueductal grey

DM

Dorsomedial hypothalamic nucleus

DMPAG

Dorsomedial periaqueductal grey

DMTg

Dorsomedial tegmental nucleus

DP

Dorsal peduncular cortex

DpMe

Deep mesencephalic nucleus

DRC

Dorsal raphe nucleus, caudal part

DRI

Dorsal raphe nucleus, interfascicular part

DTg

Dorsal tegmental nucleus

DTgP

Dorsal tegmental nucleus, pericentral part

DTM

Dorsal tuberomammillary nucleus

DTT

Dorsal tenia tecta

ECu

External cuneate nucleus

EW

Edinger–Westphal nucleus

f

Fornix

F

Nucleus of the fields of Forel

FG

Fluorogold

fi

Fimbria of the hippocampus

fmi

Forceps minor of the corpus callosum

fr

Fasciculus retroflexus

GrDG

Granular layer of the dentate gyrus

Gus

Gustatory thalamic nucleus

HDB

Nucleus of the horizontal limb of the diagonal band

I

Intercalated nuclei of the amygdala

IAD

Interanterodorsal thalamic nucleus

ic

Internal capsule

ICj

Islands of Calleja

ICjM

Islands of Calleja, major island

ICjvm

Islands of Calleja, ventromedial island

icp

Inferior cerebellar peduncle

IG

Indusium griseum

IL

Infralimbic cortex

IM

Intercalated amygdaloid nucleus, main part

In

Intercalated nucleus of the medulla

IO

Inferior olive

IOD

Inferior olive, dorsal nucleus

IPAC

Interstitial nucleus of the posterior limb of the anterior commissure

IPACL

Lateral interstitial nucleus of the posterior limb of the anterior commissure

IPACM

Medial interstitial nucleus of the posterior limb of the anterior commissure

IRt

Intermediate reticular nucleus

KF

Kölliker-Fuse nucleus

La

Lateral amygdaloid nucleus

LA

Lateroanterior hypothalamic nucleus

LaDL

Lateral amygdaloid nucleus, dorsolateral part

LaVL

Lateral amygdaloid nucleus, ventrolateral part

LaVM

Lateral amygdaloid nucleus, ventromedial part

LC

Locus coeruleus

LDTg

Laterodorsal tegmental nucleus

LDTgV

Laterodorsal tegmental nucleus, ventral part

LEnt

Lateral entorhinal cortex

LGP

Lateral globus pallidus

LH

Lateral hypothalamic area

LHb

Lateral habenular nucleus

lo

Lateral olfactory tract

LPBE

Lateral parabrachial nucleus, external part

LPBS

Lateral parabrachial nucleus, superior part

LPBV

Lateral parabrachial nucleus, ventral part

LPMR

Lateral posterior thalamic nucleus, mediodorsal part

LPO

Lateral preoptic area

LRt

Lateral reticular nucleus

LS

Lateral septum

LSD

Lateral septal nucleus, dorsal part

LSI

Lateral septal nucleus, intermediate part

LSV

Lateral septal nucleus, ventral part

LV

Lateral ventricle

maopt

Medial accessory optic tract

MCLH

Magnocellular nucleus of the lateral hypothalamus

MCPO

Magnocellular preoptic nucleus

MD

Mediodorsal thalamic nucleus

MdD

Medullary reticular nucleus, dorsal part

MdV

Medullary reticular nucleus, ventral part

Me

Medial amygdaloid nucleus

Me5

Mesencephalic trigeminal nucleus

MeA

Medial amygdaloid nucleus, anterior part

MeAD

Medial amygdaloid nucleus, anterior dorsal part

MeAV

Medial amygdaloid nucleus, anteroventral part

MePD

Medial amygdaloid nucleus, posterodorsal part

MePV

Medial amygdaloid nucleus, posteroventral part

mfb

Medial forebrain bundle

MGP

Medial globus pallidus (entopeduncular nucleus)

MHb

Medial habenular nucleus

ml

Medial lemniscus

mlf

Medial longitudinal fasciculus

MnPO

Median preoptic nucleus

MPA

Medial preoptic area

MPB

Medial parabrachial nucleus

MPO

Medial preoptic nucleus

MPOL

Medial preoptic nucleus, lateral part

MPOM

Medial preoptic nucleus, medial part

MS

Medial septal nucleus

mt

Mammillothalamic tract

mtg

Mammillotegmental tract

MTu

Medial tuberal nucleus

MVe

Medial vestibular nucleus

mvStP

Medioventral striato-pallidum

NADPHd

Nicotinamide adenine dinucleotide phosphate diaphorase

ns

Nigrostriatal bundle

O

Nucleus O

opt

Optic tract

OT

Oxytocin

OT-ir

Oxytocin-like immunoreactive (referring to immunostaining using antibodies raised against oxytocin or against its specific neurophysin)

OTR

Oxytocin receptor

PaAP

Paraventricular hypothalamic nucleus, anterior parvicellular

PAG

Periaqueductal grey

PaL

Paraventricular hypothalamic nucleus, lateral part

PaM

Paraventricular hypothalamic nucleus, medial part

PaPo

Paraventricular hypothalamic nucleus, posterior part

PaV

Paraventricular hypothalamic nucleus, ventral part

PB

Phosphate buffer

PBS

Phosphate-buffered saline

PC

Paracentral thalamic nucleus

Pc

Posterior commissure

PCom

Nucleus of the posterior commissure

Pe

Periventricular hypothalamic nucleus

PeF

Perifornical nucleus

PF

Parafascicular thalamic nucleus

PH

Posterior hypothalamic area

Pir

Piriform cortex

PLCo

Posterolateral cortical amygdaloid nucleus

PMCo

Posteromedial cortical amygdaloid nucleus

PMD

Premammillary nucleus, dorsal part

PMn

Paramedian reticular nucleus

PMnR

Paramedian raphe nucleus

PMV

Premammillary nucleus, ventral part

PnC

Pontine reticular nucleus, caudal part

PO

Periolivary region

Pr5

Principal sensory trigeminal nucleus

PRh

Perirhinal cortex

PrL

Prelimbic cortex

PSTh

Parasubthalamic nucleus

PT

Paratenial thalamic nucleus

PV

Paraventricular thalamic nucleus

PVA

Paraventricular thalamic nucleus, anterior part

Py

Pyramidal cell layer of the hippocampus

Rad

Stratum radiatum of the hippocampus

Re

Reuniens thalamic nucleus

RI

Rostral interstitial nucleus of the medial longitudinal fasciculus

RLi

Rostral linear nucleus of the raphe

RMg

Raphe magnus nucleus

RPF

Retroparafascicular nucleus

RRF

Retrorubral field

rs

Rubrospinal tract

Rt

Reticular thalamic nucleus

SCh

Suprachiasmatic nucleus

SCO

Subcommissural organ

scp

Superior cerebellar peduncle

SFi

Septofimbrial nucleus

SFO

Subfornical organ

Shi

Septohippocampal nucleus

SHy

Septohypothalamic nucleus

SI

Substantia innominata

SL

Semilunar nucleus

SLu

Stratum lucidum, hippocampus

SM

Nucleus of the stria medullaris

sm

Stria medullaris of the thalamus

SN

Substantia nigra

Sol

Nucleus of the solitary tract

sol

Solitary tract

SON

Supraoptic nucleus

SOR

Supraoptic nucleus, retrochiasmatic part

sp5

Spinal trigeminal tract

st

Stria terminalis

StA

Striatal part of the preoptic area

Su3

Supraoculomotor periaqueductal grey

Su5

Supratrigeminal nucleus

SubB

Subbrachial nucleus

SubC

Subcoeruleus nucleus

TBS

TRIS-buffered saline

TC

Tuber cinereum area

Te

Terete hypothalamic nucleus

Tu

Olfactory tubercle

Unc

Uncinate fasciculus

V1aR

Arginine vasopressin receptor type 1a

V1bR

Arginine vasopressin receptor type 1b

VA

Ventral anterior thalamic nucleus

VDB

Nucleus of the vertical limb of the diagonal band

VEn

Ventral endopiriform nucleus

VLPO

Ventrolateral preoptic nucleus

VM

Ventromedial thalamic nucleus

VMH

Ventromedial hypothalamic nucleus

VMHC

Ventromedial hypothalamic nucleus, central part

VMHDM

Ventromedial hypothalamic nucleus, dorsomedial part

VMHVL

Ventromedial hypothalamic nucleus, ventrolateral part

VMPO

Ventromedial preoptic nucleus

VOLT

Vascular organ of the lamina terminalis

VP

Ventral pallidum

vsc

Ventral spinocerebellar tract

VTA

Ventral tegmental area

VTg

Ventral tegmental nucleus

VTM

Ventral tuberomammillary nucleus

VTT

Ventral tenia tecta

Xi

Xiphoid thalamic nucleus

ZI

Zona incerta

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Marcos Otero-García
    • 2
  • Carmen Agustín-Pavón
    • 1
  • Enrique Lanuza
    • 2
  • Fernando Martínez-García
    • 1
  1. 1.Lab. of Functional Neuroanatomy (NeuroFun), Unitat Predepartamental de Medicina, Facultat de Ciències de la SalutUniversitat Jaume ICastelló de la PlanaSpain
  2. 2.Departaments de Biologia Cel·lular i de Biologia Funcional, Facultat de Ciències BiològiquesUniversitat de ValènciaValènciaSpain

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