Ryanodine-mediated conversion of STP to LTP is lacking in synaptopodin-deficient mice
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In previous studies we and others have found that activation of ryanodine receptors (RyRs) facilitate expression of long-term potentiation (LTP) of reactivity to afferent stimulation in hippocampal slices, with a more pronounced action in the ventral hippocampus. We have also been able to link the involvement of synaptopodin (SP), an actin-binding protein, with neuronal plasticity via its interaction with RyRs. To test this link more directly, we have now compared the ability of ryanodine to convert short-term to LTP in hippocampal slices taken from normal and SP-knockout (SPKO) mice. Indeed, SPKO hippocampus expresses lower concentrations of RyRs and in slices of these mice ryanodine is unable to facilitate conversion of short-term to LTP. These observations link functionally SP with calcium stores.
KeywordsSynaptopodin Calcium stores LTP Ryanodine receptors Hippocampus
Supported by a grant from German/Israel Foundation #1088-21/2010 to MS. We wish to thank Ms. E. Biton for the immunostaining, and Ms. S. Dayan for participating in the immunohistochemical analysis.
Conflict of interests
The authors declare no conflict of interest.
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