Brain Structure and Function

, Volume 219, Issue 1, pp 293–302

Physical exercise increases GFAP expression and induces morphological changes in hippocampal astrocytes

  • Lisiani Saur
  • Pedro Porto Alegre Baptista
  • Priscylla Nunes de Senna
  • Mariana Fontoura Paim
  • Patricia do Nascimento
  • Jocemar Ilha
  • Pamela Brambilla Bagatini
  • Matilde Achaval
  • Léder Leal Xavier
Original Article

DOI: 10.1007/s00429-012-0500-8

Cite this article as:
Saur, L., Baptista, P.P.A., de Senna, P.N. et al. Brain Struct Funct (2014) 219: 293. doi:10.1007/s00429-012-0500-8

Abstract

Physical exercise has an important influence on brain plasticity, which affects the neuron–glia interaction. Astrocytes are susceptible to plasticity, and induce and stabilize synapses, regulate the concentration of various molecules, and support neuronal energy metabolism. The aim of our study was to investigate whether physical exercise is capable of altering the morphology, density and expression of glial fibrillary acidic protein (GFAP) in astrocytes from the CA1 region of rat hippocampus. Thirteen male rats were divided in two groups: sedentary (n = 6) and exercise (n = 7). The animals in the exercise group were submitted to a protocol of daily physical exercise on a treadmill for four consecutive weeks. GFAP immunoreactivity was evaluated using optical densitometry and the morphological analyses were an adaptation of Sholl’s concentric circles method. Our results show that physical exercise is capable of increasing the density of GFAP-positive astrocytes as well as the regional and cellular GFAP expression. In addition, physical exercise altered astrocytic morphology as shown by the increase observed in the degree of ramification in the lateral quadrants and in the length of the longest astrocytic processes in the central quadrants. Our data demonstrate important changes in astrocytes promoted by physical exercise, supporting the idea that these cells are involved in regulating neural activity and plasticity.

Keywords

Physical exercise Astrocytes GFAP Hippocampus Sholl 

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Lisiani Saur
    • 1
  • Pedro Porto Alegre Baptista
    • 1
  • Priscylla Nunes de Senna
    • 2
  • Mariana Fontoura Paim
    • 1
  • Patricia do Nascimento
    • 2
  • Jocemar Ilha
    • 1
  • Pamela Brambilla Bagatini
    • 2
  • Matilde Achaval
    • 2
  • Léder Leal Xavier
    • 1
  1. 1.Departamento de Ciências Morfofisiológicas, Laboratório de Biologia Celular e Tecidual, Faculdade de BiociênciasPontifícia Universidade Católica do Rio Grande do Sul (PUCRS)Porto AlegreBrazil
  2. 2.Departamento de Ciências Morfológicas, Laboratório de Histofisiologia Comparada, Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do Sul.Porto AlegreBrazil

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