Brain Structure and Function

, Volume 217, Issue 2, pp 337–351 | Cite as

Differential neuronal changes in medial prefrontal cortex, basolateral amygdala and nucleus accumbens after postweaning social isolation

  • Yu-Chun Wang
  • Ue-Cheung Ho
  • Meng-Ching Ko
  • Chun-Chieh Liao
  • Li-Jen Lee
Original Article

Abstract

The mesocorticolimbic system contains dopamine (DA)-producing neurons in the ventral tegmental area (VTA) and their projection targets, including the medial prefrontal cortex (mPFC), amygdala (AMY) and nucleus accumbens (NAc). Disruption of this system might attribute to mental illnesses. In the present study, we adopted the postweaning social isolation paradigm to model neuropsychiatric disorders and studied the functional and structural changes of the mesocorticolimbic system. After 8–9 weeks of isolation, rats exhibited hyperlocomotor activity and impaired sensorimotor gating compared to group-reared controls. However, the number of tyrosine hydroxylase-positive VTA neurons and the volume of VTA were not affected. Comparing with group-reared controls, the DA levels in the isolation-reared were not altered in the VTA, mPFC and NAc but decreased in the AMY. In the structural aspect, dendritic features of layer II/III pyramidal mPFC neurons; pyramidal neurons in the basolateral nucleus of amygdala (BLA) and medium spiny neurons in the core region of the NAc (NAcc) were examined. Interestingly, the neuronal changes were region-specific. The mPFC neurons had reduced dendritic complexity, spine density and elongated terminal branches. The BLA neurons had extensive dendritic arbors with short branches but unchanged spine density. The NAcc neurons had reduced total dendritic length but the segment length and spine density remained the same. Together, the results demonstrated the structural and functional changes in the mesocorticolimbic DA system of socially isolated rats. These changes may account for the behavioral impairments in these rats and attribute to the susceptibility to mental disorders related to schizophrenia and depression.

Keywords

Prepulse inhibition Hyperlocomotor activity Tyrosine hydroxylase-positive neurons Dopamine level Dendritic arbors Dendritic spine 

Abbreviations

AMY

Amygdala

BLA

Basolateral nucleus of amygdala

CE

Coefficient of error

DA

Dopamine

DOPAC

3,4-Dihydroxy-phenylacetic acid

HPLC

High performance liquid chromatography

mPFC

Medial prefrontal cortex

NAc

Nucleus accumbens

NAcc

Core region of the nucleus accumbens

P

Postnatal day

PBS

Phosphate-buffered saline

PPI

Prepulse inhibition

SD

Sprague-Dawley

TH

Tyrosine hydroxylase

VTA

Ventral tegmental area

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yu-Chun Wang
    • 1
  • Ue-Cheung Ho
    • 1
  • Meng-Ching Ko
    • 2
  • Chun-Chieh Liao
    • 2
  • Li-Jen Lee
    • 1
    • 2
    • 3
    • 4
  1. 1.School of MedicineNational Taiwan UniversityTaipeiTaiwan
  2. 2.Graduate Institute of Anatomy and Cell Biology, College of MedicineNational Taiwan UniversityTaipeiTaiwan
  3. 3.Graduate Institute of Brain and Mind SciencesNational Taiwan UniversityTaipeiTaiwan
  4. 4.Neurobiology and Cognitive Science CenterNational Taiwan UniversityTaipeiTaiwan

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