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Brain Structure and Function

, Volume 212, Issue 1, pp 75–83 | Cite as

Three-dimensional reconstruction of the axon arbor of a CA3 pyramidal cell recorded and filled in vivo

  • Lucia Wittner
  • Darrell A. Henze
  • László Záborszky
  • György BuzsákiEmail author
Original Article

Abstract

The three-dimensional intrahippocampal distribution of axon collaterals of an in vivo filled CA3c pyramidal cell was investigated. The neuron was filled with biocytin in an anesthetized rat and the collaterals were reconstructed with the aid of a NeuroLucida program from 48 coronal sections. The total length of the axon collaterals exceeded 0.5 m, with almost 40,000 synaptic boutons. The majority of the collaterals were present in the CA1 region (70.0%), whereas 27.6% constituted CA3 recurrent collaterals with the remaining minority of axons returning to the dentate gyrus. The axon arbor covered more than two thirds of the longitudinal axis of the hippocampus, and the terminals were randomly distributed both locally and distally from the soma. We suggest that the CA3 system can be conceptualized as a single-module, in which nearby and distant targets are contacted by the same probability (similar to a mathematically defined random graph). This arrangement, in combination with the parallel input granule cells and parallel output CA1 pyramidal cells, appears ideal for segregation and integration of information and memories.

Keywords

Dentate Gyrus Granule Cell Layer Axon Initial Segment Axon Collateral Biocytin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by NIH (MH54671 to GB and NSO23945 to LZ). We would like to thank Alvaro Duque for support and teaching the use of NeuroLucida.

Supplementary material

429_2007_148_MOESM1_ESM.avi (19.3 mb)
(AVI 19783 kb)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Lucia Wittner
    • 1
    • 2
    • 3
  • Darrell A. Henze
    • 1
    • 4
  • László Záborszky
    • 1
  • György Buzsáki
    • 1
    Email author
  1. 1.Center for Molecular and Behavioral NeuroscienceRutgers, The State University of New JerseyNewarkUSA
  2. 2.Institute of Experimental MedicineHungarian Academy of SciencesBudapestHungary
  3. 3.Institute for PsychologyHungarian Academy of SciencesBudapestHungary
  4. 4.Merck Research LaboratoriesWest PointUSA

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