Anatomy and Embryology

, Volume 210, Issue 1, pp 43–49 | Cite as

Short-term effects of carbendazim on the gross and microscopic features of the testes of Japanese quails (Coturnix coturnix japonica)

Original Article


Carbendazim, a metabolite of benomyl which is widely used as a fungicide, has been found to cause testicular and epididymal damage in laboratory rats, mice and hamsters. No studies of the effects of this chemical on the reproductive organs of birds have been reported previously. This report is that of an investigation on the response of the testis of the Japanese quail to experimental administration of this chemical in sexually mature and active birds. A single dose (400 mg/kg body weight) of carbendazim was administered orally to 20 quails that were sacrificed thereafter at 5 h, 3, 8 and 13 days post-exposure, at five birds spatio-temporally. Five birds acted as control. Testis weights and seminiferous tubular diameter as well as epithelial height decreased significantly from 8 day post-exposure. Epithelial histology was remarkably disrupted, and cessation of spermatogenesis occurred at 13 day post-administration of the chemical. Degenerative changes were uniform in each testis, and not patchy or multi-focal, as previously reported in the rat. The observed histological changes in the testis, due to carbendazim, were capable of causing prolonged infertility in exposed birds.


Carbendazim effects Testis structure Quails 



A research grant by the University of Pretoria is gratefully acknowledged. The research project was approved by both the Research Committee of the Faculty of Veterinary Science and the Animal Use and Care Committee of the University.


  1. Aire TA (1997) The structure of the interstitial tissue of the active and resting avian testis. Onderstepoort J Vet Res 64: 291–299PubMedGoogle Scholar
  2. Aire TA, Malmqvist M (1979) Intraepithelial lymphocytes in the excurrent ducts of the testis of the domestic fowl (Gallus domesticus). Acta Anat 103:142–149PubMedCrossRefGoogle Scholar
  3. Akingbemi BT, Ge RS, Klinefelt GR, Gunsalus GL, Hardy MP (2000) A metabolite of methoxychlor, 2,2-bis (p-hydroxyphenyl)-1,1,1-trichloroethane, reduces testosterone biosynthesis in rat Leydig cells through suppression of steady-state mRNA levels of the cholesterol side-chain cleavage enzyme. Biol Reprod 62:571–578PubMedCrossRefGoogle Scholar
  4. Carter SD, Laskey JW (1982) Effect of benomyl on reproduction in the male rat. Toxicol Lett 11:87–94CrossRefPubMedGoogle Scholar
  5. Carter SD, Hess RA, Laskey JW (1987) The fungicide methyl 2-benzimidazole carbamate infertility in male Sprague-Dawley rats. Biol Reprod 37:709–717PubMedCrossRefGoogle Scholar
  6. Hess RA, Nakai M (2000) Histopathology of the male reproductive system induced by fungicide benomyl. Histol Histopathol 15:207–224PubMedGoogle Scholar
  7. Hess RA, Moore BJ, Linder RE, Abuel-Atta A (1991) The fungicide benomyl (methyl 1-(butylcarbamate)-2-benzimidazolecarbamate) causes testicular dysfunction by inducing sloughing of germ cells and occlusion of efferent ductules. Fund Appl Toxicol 17:733–745CrossRefGoogle Scholar
  8. Lim J, Miller MG (1997) The role of the benomyl metabolite carbendazim in benomyl-induced testicular toxicity. Toxicol Appl Pharmacol 142:401–410CrossRefPubMedGoogle Scholar
  9. Nakai M, Hess RA (1994) Morphological changes in the rat Sertoli cell induced by the microtubule poison carbendazim. Tissue Cell 26:917–927CrossRefPubMedGoogle Scholar
  10. Nakai M, Hess RA (1997) Effects of carbendazim (methyl 2-benzimidazole carbamate; MBC) on meiotic spermatocytes and subsequent spermiogenesis in the rat testis. Anat Rec 247:379–387CrossRefPubMedGoogle Scholar
  11. Nakai M, Hess RA, Moore BJ, Gutroff RF, Strader LF, Linder RE (1992) Acute and long-term effects of a single dose of the fungicide carbendazim (methyl 2-benzimidazole carbamate) on the male reproductive system in the rat testis. J Androl 13:507–518PubMedGoogle Scholar
  12. Nakai M, Hess RA, Netsu J, Nasu T (1995) Deformation of the rat Sertoli cell by oral administration of carbendazim (methyl 2-benzimidazole carbamate). J Androl 16:410–416PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Anatomy and Physiology, Faculty of Veterinary ScienceUniversity of PretoriaOnderstepoortRepublic of South Africa

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