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Anatomy and Embryology

, Volume 209, Issue 2, pp 157–168 | Cite as

CD15 immunoreactivity in the developing brain of a marsupial, the tammar wallaby (Macropus eugenii)

  • K. W. S. AshwellEmail author
  • J. K. Mai
  • C. Andressen
Original Article
  • 75 Downloads

Abstract

We have studied the distribution of the CD15 epitope in the developing brain of an Australian diprotodontid metatherian mammal, the tammar wallaby (Macropus eugenii), using immunohistochemistry in conjunction with hematoxylin and eosin staining. At the time of birth (28 days after conception), CD15 immunoreactivity labeled somata in the primordial plexiform layer of the parietal cortex in a similar position to that seen in the early fetal eutherian brain. CD15 immunoreactivity in the brain of the developing pouch-young wallaby was found to be localized on the surface of radial glia at boundaries between developmentally significant forebrain compartments in a similar distribution to that seen in developing eutherian brain. These were best seen in the developing diencephalon, delineating epithalamus, ventral and dorsal thalamus and hypothalamic anlage, and in the striatum. Immunoreactivity for CD15 identified radial glia marking the lateral migratory stream at the striatopallial boundary, peaking in intensity at P19 to P25. From P37 to P54, CD15 immunoreactivity also demarcated patch compartments in the developing striatum. In contrast, CD15 immunoreactivity in hindbrain structures showed some differences from the temporospatial pattern seen in eutherian brain. These may reflect the relatively early brainstem maturation required for the newborn wallaby to be able to traverse the distance from the maternal genital tract to the pouch. The wallaby provides a convenient model for testing hypotheses concerning the role of CD15 in forebrain development because all events in which CD15 may play a critical role in forebrain morphogenesis occur during pouch life, when the young wallaby is accessible to experimental manipulation.

Keywords

Radial glia Striatum Patch/matrix compartments 

Notes

Acknowledgements

We would like to thank Dr. Lauren Marotte for providing the wallaby material, and Sabine Lensing-Höhn and Marietta Kazimirek for performing the sectioning and immunohistochemistry. Dr. M Stark developed the hybridoma-derived antibody. This work was supported in part by a grant to KWSA from the Alexander von Humboldt Foundation.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Department of Anatomy, School of Medical SciencesThe University of New South WalesSydneyAustralia
  2. 2.Department of NeuroanatomyH.-Heine University of DüsseldorfDüsseldorfGermany
  3. 3.Department of AnatomyUniversity of RostockRostockGermany

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