Virchows Archiv

, Volume 439, Issue 6, pp 798–802 | Cite as

Genetic profile of 22 pancreatic carcinoma cell lines

Analysis of K-ras, p53, p16 and DPC4/Smad4
  • Patrick S. Moore
  • Bence Sipos
  • Simonetta Orlandini
  • Claudio Sorio
  • Francisco X. Real
  • Nicholas R. Lemoine
  • Thomas Gress
  • Claudio Bassi
  • Günter Klöppel
  • Holger Kalthoff
  • Hendrik Ungefroren
  • Matthias Löhr
  • Aldo Scarpa
Original Article

Abstract.

The K-ras, p53, p16 and DPC4 genes are among those most frequently altered in pancreatic ductal carcinoma. We analyzed 22 cell lines for alterations in these genes by direct sequence analysis and methylation-specific polymerase chain reaction. These cell lines showed mutations in K-ras and p53 at frequencies of 91% and 95%, respectively. Alterations in p16INK4a were found in all cases and included nine homozygous deletions, seven mutations and promoter methylation in six cases. Eight cell lines (36%) had an alteration of DPC4, including one mutation and seven homozygous deletions. The most typical mutational profile involved K-ras, p53, and p16INK4a, concurrently aberrated in 20 cases (91%). Eight cell lines had alterations in all four genes. Inactivation of DPC4 was always accompanied by alteration of all of the other three genes. This comprehensive data regarding the cumulative genetic alterations in pancreatic carcinoma cell lines will be of great value for studies involving drug sensitivity or resistance that may be associated with inactivation of a particular gene or molecular pathway.

Pancreas Carcinoma cell lines K-ras P16 P53 DPC4/Smad4 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Patrick S. Moore
    • 1
  • Bence Sipos
    • 6
  • Simonetta Orlandini
    • 1
  • Claudio Sorio
    • 1
  • Francisco X. Real
    • 5
  • Nicholas R. Lemoine
    • 4
  • Thomas Gress
    • 3
  • Claudio Bassi
    • 2
  • Günter Klöppel
    • 6
  • Holger Kalthoff
    • 6
  • Hendrik Ungefroren
    • 6
  • Matthias Löhr
    • 7
  • Aldo Scarpa
    • 1
  1. 1.Department of Pathology, University of Verona, Strada Le Grazie, I-37134 VeronaItaly
  2. 2.Department of Surgery, University of VeronaItaly
  3. 3.Department of Internal Medicine I, University of UlmGermany
  4. 4.ICRF Molecular Oncology Unit, Department of Cancer Medicine, Imperial College School of Medicine, LondonUK
  5. 5.Unitat de Biologia Cellular i Molecular, Institut Municipal d'Investigació Mèdica, BarcelonaSpain
  6. 6.Department of Pathology and Molecular Oncology of Clinic of General Surgery University of KielGermany
  7. 7.IV Department of Internal Medicine, Medical Faculty of Mannheim, University of Heidelberg, MannheimGermany

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