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Virchows Archiv

, Volume 436, Issue 5, pp 494–497 | Cite as

Mutation of p53 with loss of heterozygosity in the osteosarcomatous component of a dedifferentiated chondrosarcoma

  • H. J. Grote
  • Regine Schneider-Stock
  • W. Neumann
  • A. Roessner
Case report

Abstract 

We investigated a dedifferentiated chondrosarcoma of a 61-year-old woman with an osteosarcomatous high-grade component for p53 alteration. The low-grade cartilaginous and the high-grade osteosarcomatous components of the tumor were macrodissected and evaluated separately by immunohistochemistry and molecular biology. We used PCR-SSCP analysis and direct sequencing to screen exons 4–8 for p53 mutations. The p53 intron 1-polymorphism was investigated for loss of heterozygosity. A functionally relevant p53 missense mutation in codon 193 of exon 6 (A-to-T transversion) with loss of wild-type allele was detected only in the dedifferentiated component. Using the monoclonal antibody DO-1, immunohistochemistry failed to show p53 overexpression. This evidence of p53 mutation may be regarded as at least a co-factor that ”switched” the preexisting low-grade conventional chondrosarcoma to a highly malignant dedifferentiated tumor.

Key words Dedifferentiated chondrosarcoma p53 mutation p53 LOH 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H. J. Grote
    • 1
  • Regine Schneider-Stock
    • 1
  • W. Neumann
    • 2
  • A. Roessner
    • 1
  1. 1.Department of Pathology, Otto-von-Guericke-University, Leipziger Strasse 44, D-39120 Magdeburg, Germany Tel.: +49-39167-15817, Fax: +49-39167-15818DE
  2. 2.Department of Orthopedics, Otto-von-Guericke-University, Magdeburg, GermanyDE

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