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Virchows Archiv

, Volume 433, Issue 2, pp 113–117 | Cite as

ILK (β1-integrin-linked protein kinase): a novel immunohistochemical marker for Ewing’s sarcoma and primitive neuroectodermal tumour

  • Doo Hyun Chung
  • Jong Im Lee
  • Myeong Cherl Kook
  • Jeong Ran Kim
  • Soon Ha Kim
  • Eun Young Choi
  • Seong Hoe Park
  • H. G. Song
ORIGINAL ARTICLE

Abstract

 ILK (β1-integrin-linked protein kinase) is a recently identified 59-kDa serine/threonine protein kinase that interacts with the cytoplasmic domain of the β1-integrin containing four ankyrin-like repeats. We have developed a polyclonal antibody against ILK and explored the ILK immunoreactivity in normal human cells and tissues. ILK was mainly expressed in cardiac muscle and skeletal muscles. Surprisingly, ILK expression was observed in Ewing’s sarcoma (ES; 100%), primitive neuroectodermal tumour (PNET; 100%), medulloblastoma (100%), and neuroblastoma (33.3%), whereas other small round cell sarcomas were not stained by the anti-ILK antibody. These results suggest that ILK could be a novel marker for tumours with primitive neural differentiation. Our findings support the notion that ES is a tumour that is closely related to PNET and that both originate from the neuroectoderm. ILK may be a sensitive and specific immunohistochemical marker and useful for the positive identification of ES and PNET in formalin-fixed, paraffin-embedded tissue sections.

Key words β1-Integrin-linked protein kinase (ILK) Ewing’s sarcoma Primitive neuroectodermal tumour Polyclonal antibody 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Doo Hyun Chung
    • 1
  • Jong Im Lee
    • 2
  • Myeong Cherl Kook
    • 1
  • Jeong Ran Kim
    • 2
  • Soon Ha Kim
    • 1
  • Eun Young Choi
    • 1
  • Seong Hoe Park
    • 1
  • H. G. Song
    • 3
  1. 1.Department of Pathology, Seoul National University College of Medicine, Seoul, KoreaKR
  2. 2.Department of Pathology, Dong Kook University College of Medicine, Kyongju, KoreaKR
  3. 3.Department of Pathology, Chungbuk National University College of Medicine, Chongju, KoreaKR

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