Virchows Archiv

, Volume 432, Issue 6, pp 529–534 | Cite as

Characterization of a newly established human chondrosarcoma cell line, CS-OKB

  • T. Chano
  • Hidetoshi Okabe
  • Yukikazu Saeki
  • Michihito Ishizawa
  • Keiji Matsumoto
  • Sinsuke Hukuda
ORIGINAL ARTICLE

Abstract

 A clonal cell line, CS-OKB, was derived from a human chondrosarcoma and characterized by cytogenetic study, immunocytochemical staining, and reverse transcriptase polymerase chain reaction (RT-PCR). Chromosomal abnormalities characteristic of malignant cartilaginous neoplasms were identified. CS-OKB cells were intensely stained with anti-type II collagen and anti-keratan sulphate antibodies. RT-PCR indicated that CS-OKB transcribes cartilage-specific genes such as type II, X procollagen, and aggrecan. This human chondrosarcoma cell line is stable and expresses well-differentiated chondrocyte-specific genes. It synthesizes well-differentiated chondrocyte-specific molecules in uncoated plastic dishes. CS-OKB may be useful for studies of human chondrocytes and in characterizing human chondrosarcomas.

Key words Human chondrosarcoma Cell line Type II collagen RT-PCR Immunocytochemistry 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • T. Chano
    • 1
  • Hidetoshi Okabe
    • 2
  • Yukikazu Saeki
    • 3
  • Michihito Ishizawa
    • 1
  • Keiji Matsumoto
    • 1
  • Sinsuke Hukuda
    • 1
  1. 1.Department of Orthopaedic Surgery, Shiga University of Medical Science, Seta, Ohtsu, Shiga 520-21-92, Japan Tel.: +81-77-548-2252, Fax: +81-77-548-2254JP
  2. 2.Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Shiga, JapanJP
  3. 3.Department of Basic Science for Health and Nursing, Shiga University of Medical Science, Shiga, JapanJP

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