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Epstein-Barr virus is absent in gastric superficial neoplastic lesions

  • Joana RibeiroEmail author
  • Mariana Malta
  • Ana Galaghar
  • Luís Pedro Afonso
  • Diogo Libânio
  • Rui Medeiros
  • Mario Dinis-Ribeiro
  • Pedro Pimentel-Nunes
  • Hugo SousaEmail author
Original Article

Abstract

Epstein-Barr virus (EBV) has been associated with about 9% of all gastric carcinomas, but its role in gastric carcinogenesis remains unclear since there is lack of evidence of EBV presence in pre-neoplastic lesions of gastric mucosa. This study intends to determine the prevalence of EBV in gastric dysplasia and superficial neoplasia to clarify whether EBV infection is an early or late event in gastric cancer development. This retrospective study included a total of 242 gastric lesions from 199 consecutive patients who were referred for endoscopic resection. The histological classification of lesions includes 137 low- and high-grade dysplasia and 105 superficial carcinomas. EBV infection was investigated by EBER-ISH. Results showed that EBV was not detected in any epithelial cells of any case with dysplasia or superficial carcinomas, although we observed the presence of a small number of EBV-infected lymphocytes in 2.1% of all lesions. These results showed that EBV is not present in gastric dysplasia neither in superficial carcinomas suggesting that EBV carcinogenesis is a late event in well/moderately differentiated gastric carcinogenesis.

Keywords

Gastric dysplasia Neoplastic lesion Gastric cancer Epstein-Barr virus (EBV) EBVaGC 

Notes

Acknowledgments

Authors would like to acknowledge the support of Portuguese League Against Cancer (LPCC-NRNorte) for the development of this project.

Author contributions

JR, HS, PPN, RM, conception and design of the study; JR and MM, EBV detection and data analysis; AG and LPA, histological review of cases and results; DL, MDR and PPN, cases selection and clinical data collection; JR, analysis and interpretation of data, drafting the manuscript, final approval; JR and HS, analysis and interpretation of data, manuscript writing; all authors were able to review the article and give final approval.

Funding Information

Joana Ribeiro was granted with a PhD Scholarship (SFRH/BD/107740/2015) from FCT-Fundação para Ciência e Tecnologia.

Compliance with ethical standards

All procedures were approved by the Ethical Committee of Portuguese Oncology Institute of Porto (CES IPO 80/2014).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Molecular Oncology & Viral Pathology Group (CI-IPOP)Portuguese Oncology Institute of PortoPortoPortugal
  2. 2.Faculty of Medicine of Porto UniversityFMUPPortoPortugal
  3. 3.Research Department, Portuguese League Against Cancer (Liga Portuguesa Contra o Cancro-Núcleo Regional do Norte)PortoPortugal
  4. 4.Department of PathologyPortuguese Oncology Institute of PortoPortoPortugal
  5. 5.Gastroenterology DepartmentPortuguese Oncology Institute of PortoPortoPortugal
  6. 6.CINTESIS - Center for Health Technology and Services Research (Centro de Investigação Médica, Faculdade de MedicinaUniversidade do PortoPortoPortugal
  7. 7.Virology ServicePortuguese Oncology Institute of PortoPortoPortugal
  8. 8.Life and Health Sciences Research Institute (ICVS), School of Health SciencesUniversity of Minho, Campus de GualtarBragaPortugal
  9. 9.ICVS/3B’s - PT Government Associate LaboratoryBraga/GuimarãesPortugal

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