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Virchows Archiv

, Volume 474, Issue 2, pp 209–217 | Cite as

Analysis of the prognostic relevance of sex-steroid hormonal receptor mRNA expression in muscle-invasive urothelial carcinoma of the urinary bladder

  • Philipp Erben
  • Danijel SikicEmail author
  • Ralph M. Wirtz
  • Thomas Martini
  • Cleo-Aron Weis
  • Johannes Breyer
  • Wolfgang Otto
  • Bastian Keck
  • Arndt Hartmann
  • Christian Bolenz
  • on behalf of the BRIDGE Consortium e.V.
Original Article
  • 38 Downloads

Abstract

Muscle-invasive urothelial carcinoma of the urinary bladder (UCB) often recurs following radical cystectomy (RC). An altered expression of sex-steroid hormone receptors has been associated with oncological outcomes of UCB and may represent therapeutic targets. Here the expression of different hormone receptors was measured on mRNA levels in patients treated by RC and associated with outcomes. Androgen receptor (AR), estrogen receptor 1 (ESR1), and progesterone receptor (PGR) mRNA expression was assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in RC samples of 87 patients with a median age of 66 (39–88) years. Univariate and multivariate analyses were performed to test associations with pathological and clinical characteristics as well as recurrence-free (RFS) and disease-specific survival (DSS). AR mRNA expression was lower in comparison with ESR1 and PGR expression (p < 0.0001). In univariate analysis, high expression levels of AR were associated with reduced RFS (HR 2.8, p = 0.015) and DSS (HR 2.8, p = 0.010). High AR mRNA expression and a positive lymph node status were independent predictors for reduced RFS (HR 2.5, p = 0.0049) and DSS (HR 3.4, p = 0.009). In patients with low AR mRNA expression, an increased ESR1 and PGR mRNA expression were associated with reduced RFS and DSS. High expression levels of AR are significantly associated with adverse outcome in patients with muscle-invasive UCB following RC. ESR1 and PGR expression status can further stratify patients with low AR expression into subgroups with significantly reduced RFS and DSS. Therapeutic targeting of AR may influence outcomes in patients with UCB.

Keywords

Androgen receptor Bladder cancer Estrogen receptor ESR Progesterone receptor Urothelial carcinoma 

Notes

Acknowledgments

The authors thank Annette Steidler, Stefanie Herlein, and Silke Claas for excellent technical support.

Philipp Erben, Danijel Sikic, Ralph M. Wirtz, Thomas Martini, Johannes Breyer, Wolfgang Otto, Bastian Keck, Arndt Hartmann, and Christian Bolenz are members of the BRIDGE Consortium e.V.

Funding

This study was funded in parts by the German Cancer Aid (Deutsche Krebshilfe (DKH)), grant number 110541.

Compliance with ethical standards

Informed consent

Informed consent was obtained from all the individual participants included in the study. The study was conducted after approval of the local ethics committee (board (Number 2013-517 N-MA/ 2016-814R-MA).

Conflict of interest

Ralph M. Wirtz is a founder of STRATIFYER Molecular Pathology GmbH. All other authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Philipp Erben
    • 1
  • Danijel Sikic
    • 2
    Email author
  • Ralph M. Wirtz
    • 3
  • Thomas Martini
    • 4
  • Cleo-Aron Weis
    • 5
  • Johannes Breyer
    • 6
  • Wolfgang Otto
    • 6
  • Bastian Keck
    • 2
  • Arndt Hartmann
    • 7
  • Christian Bolenz
    • 4
  • on behalf of the BRIDGE Consortium e.V.
  1. 1.Department of Urology, University Medical Centre Mannheim, Medical Faculty MannheimUniversity of HeidelbergMannheimGermany
  2. 2.Department of Urology and Pediatric UrologyFriedrich-Alexander University (FAU) Erlangen-NürnbergErlangenGermany
  3. 3.Stratifyer Molecular Pathology GmbHCologneGermany
  4. 4.Department of Urology and Pediatric UrologyUniversity of UlmUlmGermany
  5. 5.Institute of Pathology, University Medical Centre Mannheim, Medical Faculty MannheimUniversity of HeidelbergMannheimGermany
  6. 6.Department of UrologyUniversity of RegensburgRegensburgGermany
  7. 7.Institute of PathologyFriedrich-Alexander University (FAU) Erlangen-NürnbergErlangenGermany

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