Virchows Archiv

, Volume 473, Supplement 1, pp 1–340 | Cite as


30th European Congress of Pathology

Oral Free Paper Sessions

Sunday, 9 September 2018, 08:30 - 12:00, Room A2

OFP-01 | Joint Session: Molecular Pathology / Haematopathology


Microfluidic-based automated multiplex immunophenotyping and imaging

B. Pelz*, D. Migliozzi, G. Cappi, D. Dupouy, M. Gijs

*EPFL, Laboratory of Microsystems 2, Lausanne, Switzerland

Background & Objective: The tumour microenvironment plays a vital role in cancer development. Multiplex immunostainings allow studying the interaction of different cell types in the tumour microenvironment using a single tissue slide. The objective is to develop a fully automated microscope integrated method for rapid 10-plex fluorescent immunostaining and imaging of tissue sections.

Method:FFPE tonsil sections underwent manual dewaxing and antigen retrieval step. All subsequent steps of staining, antibody elution and imaging were automated on the microscope integrated microfluidic device. A single tissue section was stained sequentially for CD3, CD4, CD8,...

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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

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