Oral Free Paper Sessions
Sunday, 9 September 2018, 08:30 - 12:00, Room A2
OFP-01 | Joint Session: Molecular Pathology / Haematopathology
Microfluidic-based automated multiplex immunophenotyping and imaging
B. Pelz*, D. Migliozzi, G. Cappi, D. Dupouy, M. Gijs
*EPFL, Laboratory of Microsystems 2, Lausanne, Switzerland
Background & Objective: The tumour microenvironment plays a vital role in cancer development. Multiplex immunostainings allow studying the interaction of different cell types in the tumour microenvironment using a single tissue slide. The objective is to develop a fully automated microscope integrated method for rapid 10-plex fluorescent immunostaining and imaging of tissue sections.
Method:FFPE tonsil sections underwent manual dewaxing and antigen retrieval step. All subsequent steps of staining, antibody elution and imaging were automated on the microscope integrated microfluidic device. A single tissue section was stained sequentially for CD3, CD4, CD8,...