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Differential expression of E-cadherin and P-cadherin in pT3 prostate cancer: correlation with clinical and pathological features

  • Catarina Ferreira
  • João Lobo
  • Luís Antunes
  • Paula Lopes
  • Carmen Jerónimo
  • Rui Henrique
Original Article
  • 43 Downloads

Abstract

Cadherins seem to play and important role in prostate cancer (PCa) progression. E-cadherin loss of expression has been associated with poor prognosis; P-cadherin’s role is still elusive. Although pT3 PCa is often considered “high-risk cancer,” it does not exhibit an uniformly poor prognosis. Herein, we assessed the prognostic value and survival impact of E-cadherin and P-cadherin immunoexpression in pT3 PCa. Radical prostatectomy (RP) specimens from 102 pT3 PCa patients treated between 1991 and 2014 in a single institution were designated for E-cadherin and P-cadherin immunoexpression analysis. A representative block from each specimen was selected for tissue micro-array (TMA) construction, using 3 cores per case. E-cadherin immunoexpression was assessed via a digital image analysis system. For P-cadherin, scoring criteria for HER2 in gastric cancer were used. Clinical records of all patients were reviewed for baseline clinical/pathologic characteristics and follow-up data. E-cadherin-low PCa patients displayed worse disease-specific survival (DSS), although not reaching statistical significance (HR 2.65, 95%CI 0.81–7.88). However, considering the pT3b group only, those with low E-cadherin immunoexpression displayed significantly worse overall-survival (OS) and DSS (HR 3.69, 95%CI 1.18–11.50; HR 5.90, 95%CI 1.40–24.81). No significant differences in survival were found for P-cadherin differential immunoexpression. Furthermore, an association between E-cadherin and P-cadherin immunoexpression (p = 0.019) was found, as among E-cadherin-low PCa, 96.6% were P-cadherin negative. We demonstrated that low E-cadherin immunoexpression discriminates among pT3b PCa patients those with poorer survival and which might benefit from specific therapy. The role of P-cadherin in PCa seems context-dependent deserving further investigation.

Keywords

Prostate cancer E-cadherin P-cadherin Survival 

Notes

Authors Contributions

CF, JL, and RH designed the study; CF, JL, and PL performed technical procedures and acquired pathological and clinical data; CF, JL, CJ and RH analyzed and interpreted data; CF, JL, and LA performed statistical analysis; CF drafted the manuscript; all authors reviewed and approved the final version of the manuscript. All individuals listed as co-authors of the manuscript have significantly contributed to this study.

Funding

This study was supported by project CI-IPOP-17-2015 (Epigenetic signature of prostate cancer stem cells) funded by the Research Centre of Portuguese Oncology Institute of Porto. JL is supported by an FCT—Fundação para a Ciência e Tecnologia—fellowship (grant number SFRH/BD/132751/2017).

Compliance with ethical standards

This study was approved by the institutional ethics committee of Portuguese Oncology Institute Porto (Comissão de Ética para a Saúde do IPO Porto—CES 235/2017).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

428_2018_2406_MOESM1_ESM.png (23 kb)
ESM 1 Cohort stratification according to WHO prognostic grade groups, concerning disease-specific survival (PNG 23 kb)
428_2018_2406_MOESM2_ESM.docx (42 kb)
ESM 2 (DOCX 42 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of PathologyPortuguese Oncology Institute of Porto (IPOP)PortoPortugal
  2. 2.Cancer Biology and Epigenetics GroupResearch Center of Portuguese Oncology Institute of Porto (GEBC CI-IPOP)PortoPortugal
  3. 3.Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel SalazarUniversity of Porto (ICBAS-UP)PortoPortugal
  4. 4.Department of EpidemiologyPortuguese Oncology Institute of Porto (IPOP)PortoPortugal

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