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Mesothelin expression has prognostic value in stage ΙΙ/ΙΙΙ colorectal cancer

  • Takehiro Shiraishi
  • Eiji Shinto
  • Satsuki Mochizuki
  • Hitoshi Tsuda
  • Yoshiki Kajiwara
  • Koichi Okamoto
  • Takahiro Einama
  • Kazuo Hase
  • Hideki Ueno
Original Article
  • 21 Downloads

Abstract

Mesothelin (MSLN) is a cell-surface glycoprotein present on mesothelial cells and in many cancers, where expression is generally associated with an unfavorable prognosis. The clinical significance and pathological characteristics of MSLN expression were evaluated by immunohistochemical staining of tissues from 530 stage ΙΙ/ΙΙΙ colorectal cancer (CRC) patients with R0 resection. Eighty-eight (16.6%) were MSLN-positive; 33 (37.5%) showed a luminal staining pattern whereas 55 (62.5%) showed a non-luminal staining pattern. MSLN expression, including the luminal and non-luminal staining patterns, was associated with shorter cancer-specific survival (CSS) period in stage ΙΙ (n = 314, P = 0.024) and stage ΙΙΙ (n = 216, P = 0.0002) CRC patients. The non-luminal staining pattern was correlated with poor prognosis in stage ΙΙ (P = 0.0006) and III (P < 0.0001) CRC, but a luminal staining pattern was not significantly correlated with prognosis. Cox’s multivariate analysis revealed that a non-luminal staining pattern was associated with CSS independently of other conventional parameters in stage II (P = 0.040, hazard ratio (HR) = 2.92) and III (P = 0.020, HR = 2.13) CRC patients. Immunohistochemical evaluation of MSLN expression was helpful in the prediction of patient prognosis in stage II/III CRC.

Keywords

Colorectal cancer Mesothelin Glycoprotein Immunohistochemistry Prognosis 

Abbreviations

CI

Confidence interval

CSS

Cancer-specific survival

EGFR

Epidermal growth factor receptor

HR

Hazard ratio

ROC

Receiver operating characteristic

SAGE

Serial analysis of gene expression

Notes

Authors’ contributions

TS and ES conceived and designed the experiments. TS and SM performed the experiments. TS, ES, and HT analyzed histopathological data. TS and ES drafted the manuscript. HT, YK, KO, KH, and HU revised the manuscript. TS finalized the manuscript. All authors reviewed and approved the manuscript.

Compliance with ethical standards

The experiments reported here were carried out in agreements with the Declaration of Helsinki principles and in agreement with the Ethics Committee of the National Defense Medical College Hospital, Tokorozawa, Japan.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Takehiro Shiraishi
    • 1
  • Eiji Shinto
    • 1
  • Satsuki Mochizuki
    • 1
  • Hitoshi Tsuda
    • 2
  • Yoshiki Kajiwara
    • 1
  • Koichi Okamoto
    • 1
  • Takahiro Einama
    • 1
  • Kazuo Hase
    • 1
  • Hideki Ueno
    • 1
  1. 1.Department of SurgeryNational Defense Medical CollegeSaitamaJapan
  2. 2.Department of Basic PathologyNational Defense Medical CollegeSaitamaJapan

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