Factors that may influence polymorphous low-grade adenocarcinoma growth
- 241 Downloads
There is mounting evidence on the importance of some biological processes in tumor growth, such as vascular supply, apoptosis, autophagy, and senescence. We have investigated these processes in polymorphous low-grade adenocarcinoma (PLGA), in an attempt to identify those that are relevant for this particular lesion. We analyzed 31 cases of PLGA using immunohistochemistry to antibodies against CD34 and CD105 to detect blood vessels; against D2-40 to detect lymphatic vessels; against Bax, Bcl-2, and survivin to explore cell apoptosis; and against Beclin and LCB3 to investigate autophagy and against p21 and p16 to assess senescence. Our results showed that PLGA growth does not depend on newly formed vessels but only on preexisting vasculature. Furthermore, PLGA is promoted by autophagy, sustained by both anti-apoptotic and anti-senescence signals, and stimulated by Bcl-2 and survivin.
KeywordsPolymorphous low-grade adenocarcinoma Angiogenesis Apoptosis Autophagy Senescence Bcl2 Survivin LC3B
The authors would like to thank Jeruza Bossonaro and Nadir Freitas for their technical expertise.
All authors contributed equally to the preparation of the article.
Compliance with ethical standards
This study was approved by the Ethics Committee of the São Leopoldo Mandic Institute and Research Center (number 916.794).
The authors are also extremely grateful to FAPESP (São Paulo Research Foundation) and CNPq for their financial support (FAPESP #2015/12418-5 and CNPQ #304031/2014-3).
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Luna MA, Wenig BM (2005) Polymorphous low-grade adenocarcinoma. In: Barnes L, Eveson JW, Reichart P, Sidransky D, Ed. Pathol. Genet. Head Neck Tumours. pp 223–224Google Scholar