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Virchows Archiv

, Volume 469, Issue 5, pp 523–532 | Cite as

Distinct pathways of pathogenesis of intraductal oncocytic papillary neoplasms and intraductal papillary mucinous neoplasms of the pancreas

  • Olca Basturk
  • Sun M. Chung
  • Ralph H. Hruban
  • N. Volkan Adsay
  • Gokce Askan
  • Christine Iacobuzio-Donahue
  • Serdar Balci
  • Sui Y. Zee
  • Bahar Memis
  • Jinru Shia
  • David S. Klimstra
Original Article

Abstract

Intraductal oncocytic papillary neoplasm (IOPN) of the pancreas is classified as a variant of intraductal papillary mucinous neoplasm (IPMN) in the WHO guidelines. However, the neoplastic cells of IOPNs are unique, with distinctive architecture/oncocytic cytoplasm. Although molecular/immunohistochemical features of other IPMN variants have been extensively studied, those of IOPNs have not been well characterized. Expression profile of antibodies associated with genetic alterations previously described for ductal adenocarcinomas (DAs) and IPMNs (SMAD4/β-catenin/p53/mesothelin/claudin-4) as well as antibodies to mucins and differentiation markers [MUC1/MUC2/MUC5AC/MUC6/CDX2/hepatocyte paraffin-1 (HepPar-1)] was investigated in 24 IOPNs and 22 IPMNs to assess the similarities/differences between these tumors. Expression of mesothelin and claudin-4 was dissimilar between these tumor types: A higher proportion of IOPNs labeled with mesothelin [21/24 (87.5 %) of IOPNs, 6/22 (27 %) of IPMNs, p < 0.001], while the reverse was true for claudin-4 [2/23 (9 %) of IOPNs, 9/22 (41 %) of IPMNs, p = 0.01]. The results of immunolabeling for SMAD4/β-catenin/p53 were similar in both: None of the cases showed SMAD4 loss in the intraductal components, and only 1/21 (5 %) of IOPNs and 2/22 (9 %) of IPMNs revealed abnormal β-catenin expression (p = 0.49). Nuclear p53 accumulation was seen mostly in architecturally complex/high-grade dysplasia areas in both. Immunolabeling for MUC proteins showed that almost all lesions expressed MUC5AC. Twelve of the 24 (50 %) IOPNs and 6/22 (27 %) of IPMNs (p = 0.11) labeled for MUC1, whereas 7/24 (29 %) of IOPNs and 10/22 (45 %) of IPMNs labeled for MUC2 (p = 0.25). MUC6 was expressed in 8/9 (89 %) of IOPNs (strong) and 6/21 (29 %) of IPMNs (weak) (p = 0.002). Fourteen of the 23 (61 %) IOPNs and 4/22 (18 %) of IPMNs labeled for HepPar-1 (p = 0.003). These results show that IOPNs have distinct immunoprofile and provide support for the proposition that IOPN is a distinct entity developing through a mechanism different from other pancreatic ductal neoplasms.

Keywords

Pancreas IOPN IPMN Immunohistochemistry Oncocytic 

Notes

Acknowledgments

The authors thank Ms. Tanisha Daniel for her assistance during manuscript preparation and Allyne Manzo and Lorraine Corsale for their assistance with the figures.

Compliance with ethical standards

The study was performed with approval of the Institutional Review Board and in accordance with Health Insurance Portability and Accountability Act regulations.

Funding

This work has been supported by the Cancer Center Support Grant (CCSG)/Core Grant/P30 CA008748.

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Olca Basturk
    • 1
  • Sun M. Chung
    • 2
    • 3
  • Ralph H. Hruban
    • 4
  • N. Volkan Adsay
    • 5
  • Gokce Askan
    • 1
  • Christine Iacobuzio-Donahue
    • 1
  • Serdar Balci
    • 5
  • Sui Y. Zee
    • 6
  • Bahar Memis
    • 5
  • Jinru Shia
    • 1
  • David S. Klimstra
    • 1
  1. 1.Department of PathologyMemorial Sloan Kettering Cancer CenterNew YorkUSA
  2. 2.Department of PathologyWeill Medical College of Cornell UniversityNew YorkUSA
  3. 3.Montefiore Medical CenterBronxUSA
  4. 4.Department of PathologyJohns Hopkins University HospitalsBaltimoreUSA
  5. 5.Department of PathologyEmory University School of MedicineAtlantaUSA
  6. 6.Department of PathologyStony Brook MedicineStony BrookUSA

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