Virchows Archiv

, Volume 456, Issue 3, pp 245–250 | Cite as

Mucosal expression of claudins 2, 3 and 4 in proximal and distal part of duodenum in children with coeliac disease

  • Dorottya Nagy Szakál
  • Hajnalka Győrffy
  • András Arató
  • Áron Cseh
  • Kriszta Molnár
  • Mária Papp
  • Antal Dezsőfi
  • Gábor Veres
Original Article

Abstract

Duodenal biopsy is an important tool to diagnose coeliac disease (CD); however, the most reliable location of biopsy site is still questionable. Claudins (CLDNs), members of a large family of adherent junction proteins, show characteristic expression pattern in inflammatory disorders; nevertheless, CLDN expression in CD is unknown. This is a comparative study to examine the CLDN 2, 3 and 4 expressions in proximal and distal part of duodenum in children with CD and in controls. Thirty-three children with newly diagnosed CD were enrolled. Fourteen healthy children served as controls. Biopsies from proximal and distal part of duodenum were taken for routine histological analysis. Immunohistochemistry were used to detect CD3+ intraepithelial lymphocytes and CLDN 2, 3 and 4 protein expressions. Macroscopic picture, routine histology and Marsh grade depicted no differences between biopsies taken from proximal or distal part of duodenum. However, CLDN 2 expression was significantly increased in severe form of coeliac disease in bulb and in distal duodenum, and in distal part of non-severe coeliac patients, in comparison to controls. Similar association was found concerning CLDN 3 expression. Expression of CLDN 4 was similar in all groups studied. Both proximal and distal mucosal duodenal biopsies are suitable for diagnosing villous atrophy in patients with CD. Increased expressions of CLDN 2 and 3 suggest structural changes of tight junction in coeliac disease which may be, at least in part, responsible for increased permeability and proliferation observed in coeliac disease.

Keywords

Claudin Immunohistochemistry Duodenum Biopsy Coeliac disease 

Notes

Acknowledgements

This work was supported by grants NKFP-1A/0023/2002 from Hungarian Ministry of Health, National Research Development Projects; OTKA T 049559 from the National Science Research Foundation and ETT-077/2003 from the Hungarian Ministry of Health.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Dorottya Nagy Szakál
    • 1
  • Hajnalka Győrffy
    • 2
  • András Arató
    • 1
  • Áron Cseh
    • 1
  • Kriszta Molnár
    • 1
  • Mária Papp
    • 3
  • Antal Dezsőfi
    • 1
  • Gábor Veres
    • 1
  1. 1.1st Department of PediatricsSemmelweis UniversityBudapestHungary
  2. 2.2nd Department of PathologySemmelweis Medical UniversityBudapestHungary
  3. 3.2nd Department of MedicineUniversity of DebrecenDebrecenHungary

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