Virchows Archiv

, Volume 455, Issue 1, pp 49–54 | Cite as

Differential expression of microRNA 181b and microRNA 21 in hyperplastic polyps and sessile serrated adenomas of the colon

  • Klaus Juergen Schmitz
  • Sascha Hey
  • Anja Schinwald
  • Jeremias Wohlschlaeger
  • Hideo Andreas Baba
  • Karl Worm
  • Kurt Werner Schmid
Original Article

Abstract

This study was designed to analyse the potential diagnostic value of miR-181b and miR-21 for discriminating hyperplastic polyps (HP) from sessile serrated adenomas (SSA) without cytologic dysplasia. Using real-time polymerase chain reaction expression levels of miR-181b and miR-21 in 18 HPs, 19 SSAs without cytologic dysplasia and 20 normal colonic mucosal specimens were examined. In addition, 20 colorectal cancers specimen were analysed for miR-181b expression. Data were normalised to RNU48 as an internal control. A differential expression of miR-181b and miR-21 was found in HPs, SSAs, and normal colonic mucosa with highest expression levels in SSAs. Levels of miR-181b but not miR-21 differed in HPs and normal mucosa. SSAs exhibited both significantly higher miR-181b levels (up to 2.01-fold; P < 0.001) and miR-21 levels (up to 1.82-fold; P = 0.011) than HPs. In contrast to HPs, SSAs are characterised by high levels of miR-181b and miR-21 expression. However, due to the overlap of values, miR-181b and miR-21 evaluation did not allow discrimination of the two lesions in every case.

Keywords

MicroRNA Hyperplastic polyp Sessile serrated adenoma Polymerase chain reaction 

Notes

Conflict of interest

We declare that we have no conflict of interest.

References

  1. 1.
    Estrada RG, Spjut HJ (1980) Hyperplastic polyps of the large bowel. Am J Surg Pathol 4:127–133PubMedCrossRefGoogle Scholar
  2. 2.
    Kinzler KW, Vogelstein B (1996) Lessons from hereditary colorectal cancer. Cell 87:159–170PubMedCrossRefGoogle Scholar
  3. 3.
    Higuchi T, Sugihara K, Jass JR (2005) Demographic and pathological characteristics of serrated polyps of colorectum. Histopathology 47:32–40PubMedCrossRefGoogle Scholar
  4. 4.
    Torlakovic E, Snover DC (1996) Serrated adenomatous polyposis in humans. Gastroenterology 110:748–755PubMedCrossRefGoogle Scholar
  5. 5.
    Makinen MJ (2007) Colorectal serrated adenocarcinoma. Histopathology 50:131–150PubMedCrossRefGoogle Scholar
  6. 6.
    Torlakovic E, Skovlund E, Snover DC et al (2003) Morphologic reappraisal of serrated colorectal polyps. Am J Surg Pathol 27:65–81PubMedCrossRefGoogle Scholar
  7. 7.
    Goldstein NS, Bhanot P, Odish E et al (2003) Hyperplastic-like colon polyps that preceded microsatellite-unstable adenocarcinomas. Am J Clin Pathol 119:778–796PubMedCrossRefGoogle Scholar
  8. 8.
    Wightman B, Ha I, Ruvkun G (1993) Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75:855–862PubMedCrossRefGoogle Scholar
  9. 9.
    Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75:843–854PubMedCrossRefGoogle Scholar
  10. 10.
    Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297PubMedCrossRefGoogle Scholar
  11. 11.
    Schetter AJ, Leung SY, Sohn JJ et al (2008) MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA 299:425–436PubMedCrossRefGoogle Scholar
  12. 12.
    Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 3:1101–1108PubMedCrossRefGoogle Scholar
  13. 13.
    Volinia S, Calin GA, Liu CG et al (2006) A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A 103:2257–2261PubMedCrossRefGoogle Scholar
  14. 14.
    Yanaihara N, Caplen N, Bowman E et al (2006) Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell 9:189–198PubMedCrossRefGoogle Scholar
  15. 15.
    Siebolts U, Varnholt H, Drebber U et al (2009) Tissues from routine pathology archives are suitable for microRNA analyses by quantitative PCR. J Clin Pathol 62:84–88PubMedCrossRefGoogle Scholar
  16. 16.
    Asangani IA, Rasheed SA, Nikolova DA et al (2008) MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene 27:2128–2136PubMedCrossRefGoogle Scholar
  17. 17.
    Tuppurainen K, Makinen JM, Junttila O et al (2005) Morphology and microsatellite instability in sporadic serrated and non-serrated colorectal cancer. J Pathol 207:285–294PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Klaus Juergen Schmitz
    • 1
  • Sascha Hey
    • 1
  • Anja Schinwald
    • 1
  • Jeremias Wohlschlaeger
    • 1
  • Hideo Andreas Baba
    • 1
    • 2
  • Karl Worm
    • 1
  • Kurt Werner Schmid
    • 1
    • 2
  1. 1.Institute of Pathology and NeuropathologyUniversity Hospital Essen, University of Duisburg-EssenEssenGermany
  2. 2.West German Cancer CenterEssenGermany

Personalised recommendations