Virchows Archiv

, Volume 454, Issue 6, pp 667–676

Microsomal prostaglandin E synthase protein levels correlate with prognosis in colorectal cancer patients

  • Tsuguhiko Seo
  • Atsushi Tatsuguchi
  • Seiichi Shinji
  • Masaoki Yonezawa
  • Keigo Mitsui
  • Shu Tanaka
  • Shunji Fujimori
  • Katya Gudis
  • Yuh Fukuda
  • Choitsu Sakamoto
Original Article

DOI: 10.1007/s00428-009-0777-z

Cite this article as:
Seo, T., Tatsuguchi, A., Shinji, S. et al. Virchows Arch (2009) 454: 667. doi:10.1007/s00428-009-0777-z

Abstract

The aim of this study is to investigate the expression of three prostaglandin E synthase (PGES) isomers in colorectal cancer (CRC) tissue and to evaluate their relationship to clinicopathological factors and patient prognosis. Microsomal PGES (mPGES)-1, mPGES-2, cytosolic PGES (cPGES) and cyclooxygenase (COX)-2 protein expression were analyzed by real-time polymerase chain reaction and Western blot. The localization of each PGES and COX-2 protein was examined by immunohistochemistry in 155 surgical resections and correlated to clinicopathological factors and patient prognosis. mPGES-1 mRNA and protein levels were significantly higher in CRC than in paired normal tissues. mPGES-1 immunoreactivity localized in cancer cells in 43% of cases. mPGES-2 immunoreactivity was significantly more pronounced in cancer cells than in adjacent normal epithelium in 36% of cases. cPGES immunoreactivity was homogeneous in cancer cells and thus determined constitutive. mPGES-1 and mPGES-2 correlated with significantly worse prognosis in stage I–III patients. These results indicate that mPGES-1 and mPGES-2 may each play a role in CRC progression.

Keywords

Colorectal neoplasm Cyclooxygenase-2 Prognosis Prostaglandin E synthase Immunohistochemistry 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Tsuguhiko Seo
    • 1
  • Atsushi Tatsuguchi
    • 1
    • 2
  • Seiichi Shinji
    • 3
  • Masaoki Yonezawa
    • 1
  • Keigo Mitsui
    • 1
  • Shu Tanaka
    • 1
  • Shunji Fujimori
    • 1
  • Katya Gudis
    • 1
  • Yuh Fukuda
    • 2
  • Choitsu Sakamoto
    • 1
  1. 1.Department of Medicine, Division of GastroenterologyNippon Medical SchoolTokyoJapan
  2. 2.Department of Analytic Human PathologyNippon Medical SchoolTokyoJapan
  3. 3.Surgery for Organ Function and Biological Regulation, Department of Surgery I, Graduate School of MedicineNippon Medical SchoolTokyoJapan

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