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Virchows Archiv

, Volume 453, Issue 1, pp 9–16 | Cite as

Reduced mRNA expression in paraffin-embedded tissue identifies MLH1- and MSH2-deficient colorectal tumours and potential mutation carriers

  • Annegret Müller
  • Dirk Zielinski
  • Nicolaus Friedrichs
  • Barbara Oberschmid
  • Sabine Merkelbach-Bruse
  • Hans K. Schackert
  • Markus Linnebacher
  • Magnus von Knebel Doeberitz
  • Reinhard BüttnerEmail author
  • Josef Rüschoff
  • The German HNPCC Consortium, German Cancer Aid (Deutsche Krebshilfe)
Original Article

Abstract

Based on the principle of nonsense-mediated mRNA decay, we sought to identify MLH1 or MSH2-deficient colorectal tumours through relative quantification of mRNA expression with real-time PCR (RT-PCR) analysis. MLH1 and MSH2 mRNAs were almost equally expressed as defined by MLH1 to MSH2 transcript ratio (mean 1.41) in microsatellite stable, mismatch repair (MMR) proficient tumours (n = 16). A close correlation between loss of protein expression and MMR–mRNA levels was found in highly microsatellite instable (MSI-H) tumours deficient of MLH1 or MSH2. MLH1/MSH2 ratio was low in 11 sporadic and nine hereditary MLH1-deficient carcinomas (mean 0.51), whereas the ratio was high in 17 MSH2-deficient hereditary non-polyposis colorectal cancer (HNPCC) associated carcinomas (mean 6.8). Notably, in the normal tissues of HNPCC patients with MSH2 mutations, the MLH1/MSH2 transcript ratios were significantly elevated (ratio > 2.0) as compared to the ratios of normal mucosa in patients with MMR-proficient tumours (27 of 32 ratio < 2.0; p = 0.00113). Analysis of B-lymphocytes of HNPCC patients with proven MMR gene mutation confirmed these findings. In conclusion, RT-PCR allows relative quantification of MMR gene mRNA expression in formalin-fixed and paraffin-embedded tissue. Furthermore, this approach enables quantification of haploinsufficiency due to nonsense-mediated mRNA decay in normal tissue and B-lymphocytes from patients carrying MSH2 germline mutations and may be useful for identification of asymptomatic carriers of pathogenic germline mutations.

Keywords

Diagnostic tool MMR-deficient tumours Nonsense-mediated decay qRT-PCR 

Notes

Acknowledgements

The work of the Consortium is supported by a multi-center grant from the Deutsche Krebshilfe (German Cancer Aid), Bonn, Germany. This study is in part the diploma thesis of D.Z. We thank Katja Bräutigam and Katja Goldacker for excellent technical assistance.

This work is also supported by the DFG-Grand 1304 (Klinische Forschergruppe 179)

Conflict of interest statement

We declare that we have no conflict of interest

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Annegret Müller
    • 1
    • 2
  • Dirk Zielinski
    • 2
  • Nicolaus Friedrichs
    • 3
  • Barbara Oberschmid
    • 2
  • Sabine Merkelbach-Bruse
    • 3
  • Hans K. Schackert
    • 4
  • Markus Linnebacher
    • 5
  • Magnus von Knebel Doeberitz
    • 6
  • Reinhard Büttner
    • 3
    Email author
  • Josef Rüschoff
    • 2
  • The German HNPCC Consortium, German Cancer Aid (Deutsche Krebshilfe)
  1. 1.Department of General SurgeryUniversity of GöttingenGöttingenGermany
  2. 2.Institute of Pathology Nordhessen and Targos Molecular Pathology GmbHKasselGermany
  3. 3.Institute of PathologyUniversity Hospital BonnBonnGermany
  4. 4.Department of Surgical ResearchTechnische Universität DresdenDresdenGermany
  5. 5.Institute of Molecular DiagnosticsUniversity of RostockRostockGermany
  6. 6.Institute of Molecular PathologyUniversity of HeidelbergHeidelbergGermany

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