Virchows Archiv

, Volume 453, Issue 1, pp 33–41

Re-expression of nestin in the myocardium of postinfarcted patients

  • J. Mokry
  • R. Pudil
  • J. Ehrmann
  • D. Cizkova
  • J. Osterreicher
  • S. Filip
  • Z. Kolar
Original Article
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Abstract

Intact cardiac muscle cells in the adult heart do not express intermediate filament nestin. In this study, we report on widespread expression of intermediate filament nestin in human myocardium of patients who died from the myocardial infarction. Nestin was detected in cardiomyocytes, endothelial cells, and few interstitial cells. Elevated levels of nestin were observed in cardiac muscle cells in all specimens, although the intensity of immunoreactivity and distribution of the signal differed. The strongest immunoreactivity was observed from 4 days after myocardial infarction in the infarction border zone where nestin was distributed homogeneously in the entire sarcoplasm of cardiac muscle cells. Within the following week, nestin in immunoreactive cardiomyocytes was redistributed and restricted to small subsarcolemmal foci and to intercalated discs. Angiogenic capillaries that grew between vital nestin-positive cardiomyocytes and entered the necrotic area expressed also high levels of nestin. Nestin-positive endothelial cells were often observed in mutual interactions with nestin-positive cardiac muscle cells. These findings document a crucial role of nestin in remodeling cytoskeleton of cells in the human postinfarcted myocardium.

Keywords

Human heart Nestin Cardiac muscle cells Myocardium infarction Myocardium regeneration 

Abbreviation

PCNA

proliferating cell nuclear antigen

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • J. Mokry
    • 1
    • 5
  • R. Pudil
    • 2
  • J. Ehrmann
    • 3
  • D. Cizkova
    • 1
  • J. Osterreicher
    • 4
  • S. Filip
    • 1
  • Z. Kolar
    • 3
  1. 1.Department of Histology and Embryology, Medical Faculty in Hradec KraloveCharles University in PragueHradec KraloveCzech Republic
  2. 2.Department of Internal MedicineFaculty HospitalHradec KraloveCzech Republic
  3. 3.Department of PathologyPalacky University, Medical Faculty in OlomoucOlomoucCzech Republic
  4. 4.Department of RadiobiologyFaculty of Military Health Sciences, University of DefenceHradec KraloveCzech Republic
  5. 5.Department of Histology and EmbryologyCharles University Medical FacultyHradec KraloveCzech Republic

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