Virchows Archiv

, Volume 452, Issue 6, pp 607–620 | Cite as

Inactivation of p16INK4a/CDKN2A gene may be a diagnostic feature of large B cell lymphoma leg type among cutaneous B cell lymphomas

  • Marc-Antoine Belaud-Rotureau
  • Virginie Marietta
  • Beatrice Vergier
  • Guillaume Mainhaguiet
  • Michelle Turmo
  • Yamina Idrissi
  • Jacky Ferrer
  • Marie Beylot-Barry
  • Pierre Dubus
  • Jean-Philippe Merlio
Original Article

Abstract

The World Health Organization–European Organization for Research and Treatment of Cancer has individualized three main categories among the primary cutaneous B cell lymphoma (PCBCL): leg-type primary cutaneous large B cell lymphoma (PCLBCL leg type), primary cutaneous follicle center lymphoma (PCFCL), and primary cutaneous marginal zone lymphoma (PCMZL). The genetic features of 21 PCBCL cases (six PCLBCL leg type four PCFCL large cells, seven PCFCL small cells, and four PCMZL) were investigated by comparative genomic hybridization (CGH array). Fluorescent in situ hybridization (FISH) analysis was performed to confirm CGH array data and to detect lymphoma-associated gene rearrangements. p14ARF/p16INK4aCDKN2A gene quantification, methylation analysis, and immunohistochemical detection were also performed. CGH array showed a higher number of recurrent genetic imbalances in PCLBCL leg type (mean 62) than in PCFCL large cells (mean 34). PCFCL small cells and PCMZL exhibited fewer chromosomal alterations (mean 24 and 9). FISH analysis provided concordant results with CGH array data in 97% (98 of 101) assays and demonstrated a t(8;14)(q24;q32) in two of six PCLBCL leg type. Recurrent deletions in 9p21 (p14ARF/p16INK4aCDKN2A) were a constant finding in PCLBCL leg type (six of six). Conversely, PCFCL large cells exhibited recurrent 1p36 deletions (four of four) without deletion in 9p21 (zero of four). The diagnostic and prognostic impact of the p16INK4aCDKN2A gene status in PCBCL should therefore be confirmed on a larger series.

Keywords

B cell Lymphoma Skin Cutaneous B cell lymphoma p16 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Marc-Antoine Belaud-Rotureau
    • 1
    • 2
  • Virginie Marietta
    • 1
  • Beatrice Vergier
    • 1
    • 2
  • Guillaume Mainhaguiet
    • 1
  • Michelle Turmo
    • 1
  • Yamina Idrissi
    • 1
  • Jacky Ferrer
    • 1
    • 2
  • Marie Beylot-Barry
    • 1
    • 3
  • Pierre Dubus
    • 1
    • 2
  • Jean-Philippe Merlio
    • 1
    • 2
  1. 1.EA 2406 Histologie et Pathologie Moléculaire des TumeursUniversité Victor SegalenBordeaux CedexFrance
  2. 2.Service d’Anatomie et Cytologie PathologiquesHôpital du Haut-LévêquePessac CedexFrance
  3. 3.Service de DermatologieHôpital du Haut-LévêquePessac CedexFrance

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