Virchows Archiv

, Volume 451, Issue 6, pp 1039–1045

COX-2 localization within plasma membrane caveolae-like structures in human lobular intraepithelial neoplasia of the breast

  • Giuseppe Perrone
  • Mariagiovanna Zagami
  • Vittorio Altomare
  • Cleonice Battista
  • Sergio Morini
  • Carla Rabitti
Original Article
  • 149 Downloads

Abstract

Cyclooxygenase-2 (COX-2) is highly expressed in human intraepithelial neoplasia of the breast and takes part in the molecular pathway implicated in progression of breast cancer. Recently, we demonstrated that COX-2 protein is mainly located in plasma membrane of lobular intraepithelial neoplasia (LIN) cells suggesting a localization in caveolae-like structures. The aim of the present study is to establish subcellular locations of COX-2 and its colocalization with caveolin-1 (CAV-1) to caveolae structures in LIN. To establish a relationship between COX-2 and CAV-1, 39 LINs were studied by immunohistochemistry and confocal microscopy analysis. COX-2 and CAV-1 expression was observed respectively in 79.5 and in 94.9% of LIN studied. A positive correlation was found between membrane COX-2 staining pattern and CAV-1 expression, while no correlation was found between cytoplasm COX-2 staining pattern and CAV-1. Confocal analysis showed that COX-2 localized to plasma membrane was strictly associated to CAV-1 suggesting that an amount of COX-2 protein is placed in caveolae-like structures. Our results show that COX-2 is localized within caveolae compartment and colocalized with CAV-1 protein in LIN lesions. Because caveolae are rich in signaling molecules, this COX-2 compartment may play an important role in diverse breast cancer carcinogenesis processes.

Keywords

Lobular intraepithelial neoplasia CAV-1 COX-2 Immunohistochemistry Confocal microscopy 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Giuseppe Perrone
    • 1
  • Mariagiovanna Zagami
    • 1
  • Vittorio Altomare
    • 2
  • Cleonice Battista
    • 2
  • Sergio Morini
    • 3
  • Carla Rabitti
    • 1
  1. 1.Surgical PathologyUniversità Campus Bio-Medico di RomaRomeItaly
  2. 2.Breast Surgical UnitUniversità Campus Bio-Medico di RomaRomeItaly
  3. 3.Department of Biomedical ResearchUniversità Campus Bio-Medico di RomaRomeItaly

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