High PRL-3 expression in human gastric cancer is a marker of metastasis and grades of malignancies: an in situ hybridization study
- 142 Downloads
Phosphatase of regenerating liver (PRL)-3, encoding a 22-kD low molecular weight tyrosine phosphatase, has been reported to be associated with metastasis of colorectal carcinoma. We assessed the levels of PRL-3 mRNA expression to know whether its up-regulation was involved in progression and metastasis of gastric carcinoma. Levels of PRL-3 expression in 94 human gastric adenocarcinomas and 54 matched lymph node metastases were detected by in situ hybridization and compared with clinicopathological characteristics including prognosis. High PRL-3 expression was detected in 36.2% of primary gastric carcinoma (with nodal metastasis, 55.6%; without nodal metastasis, 10%; P < 0.001) and in 74.1% of lymph node metastases. The incidence of high PRL-3 expression in lymph node metastasis was significantly higher than in primary tumors (P < 0.044). Moreover, high expression of PRL-3 was closely associated with tumor size, lymphatic invasion, venous invasion, extent of lymph node metastasis, and tumor stage. These results suggest that high PRL-3 expression may participate in the progression and metastasis of gastric carcinoma. PRL-3 might be a novel molecular marker for aggressive gastric cancer.
KeywordsPRL-3 Gastric carcinoma in situ hybridization Lymph node metastasis
This study was supported by Grants-in-Aid for Cancer Research from the Ministry of Health, Labor and Welfare of Japan (14-7). It is also supported in part by Grants-in-Aid for Scientific Research (B) (14370070) and Exploratory Research (18659096) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. Grant from the Terry Fox Run Foundation for Cancer Research was also indebted. The authors thank to Prof. Hiroki Kuniyasu (Department of Molecular Pathology, Nara Medical University) for valuable discussion and technical assistance.
- 14.Matter WF, Estridge T, Zhang C, Belagaje R, Stancato L, Dixon J, Johnson B, Bloem L, Pickard T, Donaghue M, Acton S, Jeyaseelan R, Kadambi V, Vlahos CJ (2001) Role of PRL-3, a human muscle-specific tyrosine phosphatase, in angiotensin-II signaling. Biochem Biophys Res Commun 283:1061–1068PubMedCrossRefGoogle Scholar
- 17.Park CS, Manahan LJ, Brigati DJ (1991) Automated molecular pathology: one hour in situ hybridization. J Histotechnol 14:219–229Google Scholar