Virchows Archiv

, Volume 449, Issue 1, pp 14–23 | Cite as

Genetic similarities and differences between lobular in situ neoplasia (LN) and invasive lobular carcinoma of the breast

  • Luca Morandi
  • Gianluca Marucci
  • Maria P. Foschini
  • Maria G. Cattani
  • Annalisa Pession
  • Cristina Riva
  • Vincenzo Eusebi
Original Article


One of the most controversial issues in breast pathology is whether lobular neoplasia (LN) is a risk factor or a precursor lesion of invasive lobular carcinoma (ILC). This is consequent to the fact that no conclusive data on the biology of LN exist. Molecular studies of LN and ILC are scanty, variable, and not consistent. Clonality of 12 cases of LN and ILC present simultaneously in the same block has been studied. Cells from both lesions were obtained by microdissection and were studied for mitochondrial DNA (mtDNA), D-loop sequencing, and neighbor-joining trees. Eight of the same cases were studied with comparative genomic hybridization (CGH) array to have additional data consistent with mtDNA. In all cases, loss of heterozygosity was studied for D16S496,locus 16q22.1 related to e-cadherin. It appears that no fewer than eight cases were genetically very similar (clonal) with mtDNA. Seven of these cases appeared also clonal with CGH array. It is concluded that in the present series, LN and ILC are genetically related lesions in the majority of cases and that LN might be the precursor of ILC.


CGH array mtDNA Lobular neoplasia Invasive lobular carcinoma 



This work was supported by grants number 2002064975 and 2005063089 (COFIN) from the Ministry of University and Research (MIUR).

The authors thank Dr. Alberto Goldoni (from Medical Genetics Unit, S. Orsola-Malpighi Hospital, University of Bologna) for the bioinformatics support.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Luca Morandi
    • 1
  • Gianluca Marucci
    • 1
  • Maria P. Foschini
    • 1
  • Maria G. Cattani
    • 2
  • Annalisa Pession
    • 1
  • Cristina Riva
    • 3
  • Vincenzo Eusebi
    • 1
    • 4
  1. 1.Section of Pathology, Department of OncologyUniversity of BolognaBolognaItaly
  2. 2.Section of Pathology, Department of OncologyUniversity of BolognaBolognaItaly
  3. 3.Section of Pathology, Department of Human MorphologyUniversity of InsubriaVareseItaly
  4. 4.Anatomia PatologicaBolognaItaly

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