Virchows Archiv

, Volume 447, Issue 1, pp 53–60

Expression of BAFF-R (BR3) in normal and neoplastic lymphoid tissues characterized with a newly developed monoclonal antibody

  • Naoya Nakamura
  • Hidenori Hase
  • Daisuke Sakurai
  • Sachiko Yoshida
  • Masafumi Abe
  • Nobuhiro Tsukada
  • Jun Takizawa
  • Sadao Aoki
  • Masaru Kojima
  • Shigeo Nakamura
  • Tetsuji Kobata
Original Article

Abstract

BAFF-receptor (BAFF-R) is required for the successful maturation and survival of B-cells. We developed an anti-human BAFF-R monoclonal antibody (mAb), 8A7. The reactivity of 8A7 in normal and neoplastic tissue was examined by performing immunohistochemistry on paraffin-embedded sections. 8A7 reacted with lymphocytes in the mantle and marginal zones, but not with lymphocytes in the interfollicular area. Lymphocytes in the germinal centers were found to be negative or occasionally weakly positive for 8A7. BAFF-R expression was found only in B-cell lymphoma (44/80, positive cases/examined cases): B-lymphoblastic lymphoma 0/3, B-chronic lymphocytic leukemia/small lymphocytic lymphoma 4/4, mantle cell lymphoma 9/11, follicular lymphoma 10/14, diffuse large B-cell lymphoma (DLBCL) 11/25, marginal zone B-cell lymphoma 8/10, lymphoplasmacytic lymphoma 2/2, plasma cell myeloma 0/2, and Burkitt lymphoma 0/9, but not in T/NK cell lymphomas (0/19) or Hodgkin lymphoma (0/10). BAFF-R was expressed in most low-grade B-cell neoplasms and a small number of DLBCL, suggesting that BAFF-R may play an important role in the proliferation of neoplastic lymphoid cells. Thus, the mAb is very useful for further understanding of both healthy B-cell biology and its pathogenic neoplasms.

Keywords

BAFF BAFF-R Monoclonal antibody Immunohistochemistry B-cell lymphoma Diffuse large B-cell lymphoma 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Naoya Nakamura
    • 1
  • Hidenori Hase
    • 2
  • Daisuke Sakurai
    • 2
  • Sachiko Yoshida
    • 1
  • Masafumi Abe
    • 1
  • Nobuhiro Tsukada
    • 3
  • Jun Takizawa
    • 3
  • Sadao Aoki
    • 3
  • Masaru Kojima
    • 4
  • Shigeo Nakamura
    • 5
  • Tetsuji Kobata
    • 2
  1. 1.Department of PathologyFukushima Medical UniversityFukushima-shiJapan
  2. 2.Department of ImmunologyDokkyo University School of MedicineTochigiJapan
  3. 3.First Department of Internal Medicine, Niigata University Medical Hospital Niigata UniversityNiigataJapan
  4. 4.Department of PathologyDokkyo University School of MedicineTochigiJapan
  5. 5.Department of Pathology and Molecular DiagnosticsAichi Cancer CenterNagoyaJapan

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