Virchows Archiv

, Volume 444, Issue 2, pp 183–189 | Cite as

Pathology of the pancreas and other organs in the diabetic LEW.1AR1/Ztm-iddm rat, a new model of spontaneous insulin-dependent diabetes mellitus

  • Anne Jörns
  • Birgit Kubat
  • Markus Tiedge
  • Dirk Wedekind
  • Hans-Jürgen Hedrich
  • Günter Klöppel
  • Sigurd Lenzen
Original Article


We studied the histo- and immunopathology of the endocrine and exocrine pancreas and a number of other organs in a new insulin-dependent diabetes mellitus (IDDM) rat model (LEW.1AR1/Ztm-iddm rat). The pancreas of the acutely diabetic animals showed an inflammatory infiltrate, involving all islets and ducts. The islet infiltrate was composed mainly of ED1-positive macrophages and T lymphocytes, comprising a large number of CD8+ lymphocytes and a few CD4+ lymphocytes. In addition, the islets displayed apoptotic cells, characterized by condensation and fragmentation of nuclear chromatin. These cells were identified as beta cells by insulin immunostaining. Other endocrine and exocrine glands, including adrenals and thyroid, as well as salivary and submandibular glands, were unaffected. Organs from the digestive tract or systemic circulatory system, including small intestine, liver, heart, and lung also showed no involvement. The kidney was intact in acutely diabetic rats. However, 6 months after diabetes manifestation, pathological changes compatible with a diabetic nephropathy had developed, affecting both the glomerula and the proximal tubular segments. It was concluded that the autoimmune process in this new IDDM rat model is restricted to the endocrine pancreas and leads to apoptotic beta cell destruction.


Type-1 diabetes mellitus Animal model Rat Autoimmune disease Beta cell apoptosis Renal damage 



This work has been supported by a grant from the Deutsche Forschungsgemeinschaft (Jo-395 1–1) to A.J. and by the NIH grant 1R21AI55464–01 to S.L. and H.J.H. The technical assistance of M. Böger and U. Sommerfeld is gratefully acknowledged.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Anne Jörns
    • 1
  • Birgit Kubat
    • 2
  • Markus Tiedge
    • 5
  • Dirk Wedekind
    • 4
  • Hans-Jürgen Hedrich
    • 4
  • Günter Klöppel
    • 3
  • Sigurd Lenzen
    • 5
  1. 1.Centre of AnatomyHannover Medical SchoolHannoverGermany
  2. 2.Department of Cell BiologyHannover Medical SchoolHannoverGermany
  3. 3.Department of PathologyUniversity of KielKielGermany
  4. 4.Institute for Laboratory Animal ScienceHannover Medical SchoolHannoverGermany
  5. 5.Institute of Clinical BiochemistryHannover Medical SchoolHannoverGermany

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