Virchows Archiv

, Volume 444, Issue 1, pp 49–55 | Cite as

Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction

  • Yasushi Takagi
  • Toshio NikaidoEmail author
  • Toshihiko Toki
  • Naoko Kita
  • Makoto Kanai
  • Takashi Ashida
  • Satoshi Ohira
  • Ikuo Konishi
Original Article


Recent evidence suggests that oxidative stress is involved in the pathophysiology of preeclampsia. Using immunohistochemistry and Western blotting, we investigated the oxidative stress- and redox-related molecules, such as 8-hydroxy-2′-deoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE), thioredoxin (TRX) and redox factor-1 (ref-1) in the placenta in preeclampsia, intrauterine growth restriction (IUGR), preeclampsia + IUGR and in normal pregnancy. Using immunohistochemistry, the level of 8-OHdG was significantly higher in IUGR (P=0.012) or preeclampsia + IUGR (P=0.0021) than in normal pregnancy, while TRX expression was significantly higher in preeclampsia (P=0.045), and ref-1 expression was significantly higher in preeclampsia (P=0.017), IUGR (P=0.016) and preeclampsia + IUGR (P=0.0038) than in normal pregnancy. The levels of 4-HNE did not differ significantly between either preeclampsia or IUGR and normal pregnancy. A significant positive correlation was observed between TRX and ref-1 expressions in both normal (ρ=0.52) and complicated (ρ=0.43) pregnancies. Using Western blotting, ref-1 expression tended to be higher in complicated pregnancies than in normal pregnancy (P=0.09). These results suggest that oxidative DNA damage is increased in IUGR and that redox function is enhanced in both preeclampsia and IUGR compared with normal pregnancy.


Oxidative stress Intrauterine fetal growth restriction Redox function Placenta Preeclampsia 



The authors thank Professor Junji Yodoi, Kyoto University, for providing the antibody against thioredoxin.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Yasushi Takagi
    • 1
  • Toshio Nikaido
    • 2
    Email author
  • Toshihiko Toki
    • 1
  • Naoko Kita
    • 1
  • Makoto Kanai
    • 1
  • Takashi Ashida
    • 1
  • Satoshi Ohira
    • 1
  • Ikuo Konishi
    • 1
  1. 1.Department of Obstetrics and GynecologyShinshu University School of MedicineMatsumotoJapan
  2. 2.Department of Organ Transplants, Reconstructive Medicine and Tissue EngineeringShinshu University Graduate School of MedicineMatsumoto Japan

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