Virchows Archiv

, Volume 443, Issue 6, pp 745–751 | Cite as

Immunostaining for the tumour suppressor gene p16 product is a useful marker to differentiate melanoma metastasis from lymph-node nevus

  • Daniela Mihic-Probst
  • Parvin Saremaslani
  • Paul Komminoth
  • Philipp U. Heitz
Original Article

Abstract

Upon the introduction of extensive sampling protocols of sentinel node biopsies, pathologists are increasingly confronted with small melanoma metastases. Using conventional histology, it proves sometimes difficult or impossible to differentiate small melanoma metastases from lymph-node nevi. Loss of the tumour suppressor gene p16 has been shown to be associated with tumour progression of melanoma. We investigated nevus and melanoma cells for the presence of the product of the gene p16, using immunohistochemistry. All nevus cells, independent of their location (nodal or skin) displayed an extensive nuclear and cytoplasmic staining for p16. In contrast, all cells of melanoma metastases, except one skin metastasis, lacked nuclear staining for p16. These findings indicate that p16 is a reliable marker to distinguish lymph-node nevi from melanoma metastasis.

Keywords

Nodal nevus Metastatic melanoma Sentinel lymph node Tumour suppressor gene p16 

Notes

Acknowledgements

We thank N. Wey for photographic and computer-assisted reproductions and Dr. W. Jochum, Department of Pathology, University Hospital Zürich, for critical reading of the manuscript.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Daniela Mihic-Probst
    • 1
    • 3
  • Parvin Saremaslani
    • 1
  • Paul Komminoth
    • 2
  • Philipp U. Heitz
    • 1
  1. 1.Department of PathologyUniversity HospitalZürich
  2. 2.Institute of PathologyCantonal HospitalBadenSwitzerland
  3. 3.Department of PathologyUniversity HospitalZürichSwitzerland

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