Virchows Archiv

, Volume 443, Issue 4, pp 518–523 | Cite as

The promoter methylation status of the DNA repair gene O 6-methylguanine-DNA methyltransferase in ulcerative colitis

  • Shunji Matsumura
  • Naohide Oue
  • Reiko Ito
  • Hirofumi Nakayama
  • Yasuhiko Kitadai
  • Hiroshi Yokozaki
  • Kazuaki Chayama
  • Wataru Yasui
Original Article

Abstract

Patients with long-standing and extensive ulcerative colitis (UC) have an increased incidence of colorectal cancer (CRC). It has been reported that a DNA repair gene, O 6-methylguanine-DNA methyltransferase (MGMT) is inactivated by promoter hypermethylation in sporadic CRCs. Hence, we investigated the promoter methylation status of MGMT by methylation specific polymerase chain reaction (PCR) in a total of 67 tissue samples (61 non-cancerous tissues and 6 cancer tissues) from 24 patients with UC. Promoter hypermethylation of MGMT was detected in one well-differentiated adenocarcinoma (16.7%) of 6 cancer samples and not detected in any of adenomas and dysplasias. In non-dysplastic tissues, promoter hypermethylation of MGMT was detected in 2 (3.7%, mucosa with mild inflammation) of 54 samples. The frequency of MGMT promoter hypermethylation in UC-associated CRCs found in this study (16.7%) is obviously lower than previously reported in sporadic CRCs (39.0–42.0%). We also confirmed that 42.9% (6/14) of sporadic CRCs showed the promoter methylation. These findings indicated that promoter hypermethylation of the MGMT gene is infrequent in patients with UC, and may not closely contribute to UC-associated colorectal tumorigenesis. A different genetic pathway for tumor progression may exist between sporadic CRC and UC-associated CRC.

Keywords

DNA methylation MGMT Ulcerative colitis 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Shunji Matsumura
    • 1
  • Naohide Oue
    • 1
  • Reiko Ito
    • 1
  • Hirofumi Nakayama
    • 1
  • Yasuhiko Kitadai
    • 2
  • Hiroshi Yokozaki
    • 3
  • Kazuaki Chayama
    • 2
  • Wataru Yasui
    • 1
  1. 1.Department of Molecular PathologyHiroshima University Graduate School of Biomedical SciencesHiroshimaJapan
  2. 2.Department of Medicine and Molecular ScienceHiroshima University Graduate School of Biomedical SciencesHiroshimaJapan
  3. 3.Department of Biomedical InformaticsKobe University Graduate School of Medicine, Faculty of Medical SciencesKobeJapan

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