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Virchows Archiv

, Volume 440, Issue 5, pp 461–475 | Cite as

Expression of somatostatin receptor types 1–5 in 81 cases of gastrointestinal and pancreatic endocrine tumors

A correlative immunohistochemical and reverse-transcriptase polymerase chain reaction analysis
  • M. Papotti
  • M. Bongiovanni
  • M. Volante
  • E. Allìa
  • S. Landolfi
  • L. Helboe
  • M. Schindler
  • S. Cole
  • G. Bussolati
Original Article

Abstract.

Somatostatin receptors (SSTRs) have been extensively mapped in human tumors by means of autoradiography, reverse-transcriptase polymerase chain reaction (RT-PCR), in situ hybridization (ISH) and immunohistochemistry (IHC). We analyzed the SSTR type 1–5 expression by means of RT-PCR and/or IHC in a series of 81 functioning and non-functioning gastroenteropancreatic (GEP) endocrine tumors and related normal tissues. Moreover, we compared the results with clinical, pathological and hormonal features. Forty-six cases (13 intestinal and 33 pancreatic) were studied for SSTR 1–5 expression using RT-PCR, IHC with antibodies to SSTR types 2, 3, 5 and ISH for SSTR2 mRNA. The vast majority of tumors expressed SSTR types 1, 2, 3 and 5, while SSTR4 was detected in a small minority. Due to the good correlation between RT-PCR and IHC data on SSTR types 2, 3, and 5, thirty-five additional GEP endocrine tumors were studied with IHC alone. Pancreatic insulinomas had an heterogeneous SSTR expression, while 100% of somatostatinomas expressed SSTR5 and 100% gastrinomas and glucagonomas expressed SSTR2. Pre-operative biopsy material showed an overlapping immunoreactivity with that of surgical specimens, suggesting that the SSTR status can be detected in the diagnostic work-up. It is concluded that SSTRs 1–5 are heterogeneously expressed in GEP endocrine tumors and that IHC is a reliable tool to detect SSTR types 2, 3 and 5 in surgical and biopsy specimens.

GEP Somatostatin receptors Pancreatic islets Neuroendocrine tumor Immunohistochemistry RT-PCR 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • M. Papotti
    • 1
  • M. Bongiovanni
    • 1
  • M. Volante
    • 1
  • E. Allìa
    • 1
  • S. Landolfi
    • 1
  • L. Helboe
    • 2
  • M. Schindler
    • 3
  • S. Cole
    • 3
  • G. Bussolati
    • 1
  1. 1.Department of Biomedical Sciences and Oncology, University of Turin, Via Santena 7, 10126 Torino, ItalyItaly
  2. 2.Lundbeck, Copenhagen, DenmarkDenmark
  3. 3.Glaxo Institute of Applied Pharmacology, University of Cambridge, UKUK

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