Virchows Archiv

, Volume 441, Issue 1, pp 31–40 | Cite as

High levels of cellular retinol binding protein-1 expression in leiomyosarcoma: possible implications for diagnostic evaluation

  • Augusto Orlandi
  • Arianna Francesconi
  • Sophie Clément
  • Patricia Ropraz
  • Luigi Giusto Spagnoli
  • Giulio Gabbiani
Original Article

Abstract.

Retinoid bioavailability is regulated by the activity of specific cytoplasmic receptors. High levels of cellular retinol binding protein-1 (CRBP-1) have been documented during experimental arterial and wound-healing processes, but data concerning neoplastic smooth muscle tissues are scarce and/or controversial. This study reports that the expression of CRBP-1 is markedly higher in uterine and gastrointestinal leiomyosarcomas than in leiomyomas and normal myometrium; CRBP-1 was practically absent in normal gastrointestinal smooth muscle tissue. CRBP-1 positivity was particularly elevated in the epithelioid variant of leiomyosarcoma; it was associated with increased proliferative and apoptotic rates and inversely related to smooth muscle differentiation evaluated by α- and γ-smooth muscle actin and desmin expression. Western blotting and reverse-transcription polymerase chain reaction confirmed the observations concerning CRBP-1 and actin isoform expression and revealed higher NF-κ-Bp65 and RARα and lower Bax protein levels in leiomyosarcoma than in the other conditions. These findings document that a high CRBP-1 expression is associated with smooth muscle malignancy and suggest that CRBP-1 expression represents a new useful marker for the classification of unusual variants of smooth muscle tumors.

Actin isoforms Myometrium Retinoic acid Smooth muscle 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Augusto Orlandi
    • 1
  • Arianna Francesconi
    • 1
  • Sophie Clément
    • 2
  • Patricia Ropraz
    • 2
  • Luigi Giusto Spagnoli
    • 1
  • Giulio Gabbiani
    • 2
  1. 1.Institute of Anatomic Pathology, Tor Vergata University of Rome, ItalyItaly
  2. 2.Department of Pathology-CMU, University of Geneva, 1 Rue Michel-Servet, 1211 Geneva 4, SwitzerlandSwitzerland

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