Development Genes and Evolution

, Volume 206, Issue 4, pp 292–295 | Cite as

Multicolour whole-mount in situ hybridization to Drosophila embryos

  • G. Hauptmann
  • Thomas Gerster
TECHNICAL NOTE

Abstract

 We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.

Key words Biotin-UTP Alkaline phosphatase substrates Fast Red Fast Blue B Magenta-phos/INT 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. Hauptmann
    • 1
  • Thomas Gerster
    • 1
  1. 1.Biozentrum der Universität Basel, Abteilung Zellbiologie, Klingelbergstrasse 70, CH-4056 Basel, SwitzerlandCH

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