Development Genes and Evolution

, Volume 210, Issue 2, pp 73–81

Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins

  • L. Huang
  • X. Li
  • H. M. El-Hodiri
  • S. Dayal
  • A. H. Wikramanayake
  • W. H. Klein
Original Article

DOI: 10.1007/s004270050013

Cite this article as:
Huang, L., Li, X., El-Hodiri, H. et al. Dev Gene Evol (2000) 210: 73. doi:10.1007/s004270050013

Abstract 

Members of the Tcf/Lef family interact with β-catenin to activate programs of gene expression during development. Recently β-catenin was shown to be essential for establishing cell fate along the animal-vegetal axis of the sea urchin embryo. To examine the role of Tcf/Lef in sea urchins we cloned a Strongylocentrotus purpuratus Tcf/Lef homolog. Expression of SpTcf/Lef was maximal when β-catenin became localized to nuclei of vegetal blastomeres, consistent with its acting in combination with β-catenin to specify vegetal cell fates. Expression of a dominant-negative SpTcf/Lef inhibited primary and secondary mesenchyma, endoderm, and aboral ectoderm formation in a manner similar to that observed when nuclear accumulation of β-catenin was prevented. Our results suggest that SpTcf/Lef functions by interacting with β-catenin to specify cell fates along the sea urchin animal-vegetal axis.

Key words Sea urchins embryos β-Catenin Tcf/Lef Cell fate determination 

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • L. Huang
    • 1
  • X. Li
    • 1
  • H. M. El-Hodiri
    • 1
  • S. Dayal
    • 1
  • A. H. Wikramanayake
    • 1
  • W. H. Klein
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, M.D. Anderson Cancer Center, University of Texas, 1515 Holcombe Blvd., Houston, TX 77030, USA e-mail: wklein@mdanderson.org Tel.: +1-713-7923646 Fax: +1-713-7900329US

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