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Development Genes and Evolution

, Volume 229, Issue 5–6, pp 197–206 | Cite as

RNA-seq analysis provides insight into molecular adaptations of Andrias davidianus

  • Xiaofang Geng
  • Lu Zhang
  • Xiayan Zang
  • Jianlin Guo
  • Cunshuan XuEmail author
Original Article

Abstract

The Chinese giant salamander Andrias davidianus is regarded as an ideal model for studying local adaptations, such as longevity, tolerance to starvation, and cutaneous respiration. Transcriptome analysis is useful for studying the large and complex genomes of amphibians. Based on the coding gene set of adult A. davidianus, dozens of A. davidianus–specific genes were identified and three signaling pathway (JAK-STAT, HIF-1, and FoxO) genes were expanded as compared with other amphibians. The results of the pathway analysis of A. davidianus–specific genes indicated that the molecular adaptation of A. davidianus may have required a more rapid evolution of the immune system. Additionally, for the first time, the gene expressions in different parts of the skin tissue were compared. The results of the comparison analysis demonstrated that lateral skin could be more focused on mucus secretion, dorsal skin on immunity and melanogenesis, and abdominal skin on water and salt metabolism. This study provides the first insight into studying longevity and starvation tolerance in A. davidianus, and offers a basis for further investigation of the molecular mechanisms of adaptations in amphibians.

Keywords

Chinese giant salamander Transcriptome Molecular adaptation Longevity Starvation tolerance 

Notes

Acknowledgments

We thank Chongqing Kui Xu Biotechnology Incorporated Company for providing us Chinese giant salamanders.

Authors’ contributions

CSX and XFG designed the project. XFG, LZ, and XYZ conducted the experiments. JLG contributed the analysis tools. XFG wrote the manuscript with input from all authors. All authors worked on the final version of the manuscript.

Funding information

This work was supported by grants from the National Natural Science Foundation of China (No. 31572270 and No. 81802099), and Natural Science Foundation of Henan Province (No. 162300410144).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, School of Laboratory MedicineXinxiang Medical UniversityXinxiangChina
  2. 2.State Key Laboratory Cultivation Base for Cell Differentiation Regulation, College of Life ScienceHenan Normal UniversityXinxiangChina

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