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Development Genes and Evolution

, Volume 227, Issue 5, pp 339–353 | Cite as

Conservation of the Notch antagonist Hairless in arthropods: functional analysis of the crustacean Daphnia pulex Hairless gene

  • Ariella Zehender
  • Melanie Bayer
  • Milena Bauer
  • Bettina Zeis
  • Anette Preiss
  • Dieter MaierEmail author
Original Article

Abstract

The Notch signaling pathway is highly conserved in all animal metazoa: upon Notch receptor activation, transcription of Notch target genes is turned on by an activator complex that centers on the transcription factor CSL. In the absence of signal, CSL assembles transcriptional repression complexes that display remarkable evolutionary diversity. The major antagonist of Notch signaling in insects named Hairless was originally identified in Drosophila melanogaster. It binds to the Drosophila CSL homologue Suppressor of Hairless [Su(H)] and recruits the two general co-repressors, Groucho and C-terminal binding protein. Whereas the majority of Notch signaling components is conserved between insects and vertebrates, Hairless is found only in insects. Here, we present the analysis of the Hairless gene from Daphnia pulex and, hence, for the first time from a crustacean. Daphnia and Drosophila Hairless protein sequences are highly diverged. Known functional domains, however, the Su(H), Groucho and the C-terminal binding protein interactions domains, are well conserved. Moreover, direct binding of the Daphnia Hairless protein and the respective Drosophila interaction partners was detected, demonstrating the conservation at the molecular level. In addition, interaction between Daphnia Hairless and Drosophila Su(H) was demonstrated in vivo, as co-overexpression of the respective genes during Drosophila development resulted in the expected downregulation of Notch activity in the fly. Structural models show that the Hairless-Su(H) repressor complexes from Daphnia and Drosophila are almost indistinguishable from one another. Amino acid residues in direct contact within the Hairless-Su(H) complex are at absolutely identical positions in the two homologues.

Keywords

Notch signaling Notch antagonist Hairless Daphnia pulex Drosophila melanogaster 

Notes

Acknowledgments

We greatly acknowledge U. Gigengack, H. Mastel, S. Mohaved, and H. Reichle for the technical assistance and L. Fedoseeva for the help during a Master module. We thank the DSHB for the antisera. We are grateful to J.K. Colbourne and D. Gilbert for sharing unpublished data and R.O. Schill for classifying the European Daphnia pulex species. We thank A.C. Nagel for carefully reading the manuscript. This work was supported by Deutsche Forschungsgemeinschaft (DFG) grants MA1328/10 and MA1328/11-1 to DM.

Funding

This study was funded by the German Research Foundation DFG to DM (MA1328/10 and MA1328/11-1).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

427_2017_593_MOESM1_ESM.pdf (1.4 mb)
ESM 1 (PDF 1418 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Institute of Genetics (240)University of HohenheimStuttgartGermany
  2. 2.Department of Internal Medicine 3 – Rheumatology and ImmunologyFriedrich-Alexander-University Erlangen-Nürnberg (FAU) and University Hospital ErlangenErlangenGermany
  3. 3.Institute of ZoophysiologyUniversity of MünsterMünsterGermany

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